Purpose: The purpose of this study is to assess the effects of patient participation education on the patient safety perception, patient participation, and patient-provider relationship in ROK Military Hospital. Methods: A non-equivalent control group pretest-posttest design was used for the study. Participants were 135 inpatients (control group: 71; experimental group: 64). Patient participation education was provided both offline and online using picture cards and videos. This study was conducted in a military hospital from 27 May to 31 August 2021. Data were analyzed by the chi-square test, independent t-test, paired t-test, and Analysis of Covariance (ANCOVA) using SPSS program. Results: The results revealed that there was a statistically significant difference in patient safety perception (F=16.33, p<.001) and patient participation(F=17.14, p<.001) scores between the control and experimental groups. In addition, the degree of increase in patient safety perception and patient participation scores in the experimental group was significantly higher than those of the control group (t=-3.63, p<.001; t=-3.40, p<.001). However, there was no statistically significant difference in patient-provider relationship score between the control and experimental groups. Conclusion: Patient participation education can improve patient safety perception and patient participation, which are necessary to prevent patient safety incidents and improve the quality of care. We suggest to develop systematic and standardized patient participation education in various ways.