@article{ART002530530},
author={Sae-Ra Park and Sun-A Cho and Young-So Kim and Su-Sun An},
title={Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance},
journal={Journal of Alternatives to Animal Experiments},
issn={1975-9657},
year={2018},
volume={12},
number={1},
pages={1-7},
doi={10.23032/jaae.2018.12.1.005}
TY - JOUR
AU - Sae-Ra Park
AU - Sun-A Cho
AU - Young-So Kim
AU - Su-Sun An
TI - Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance
JO - Journal of Alternatives to Animal Experiments
PY - 2018
VL - 12
IS - 1
PB - Korean Society for Alternatives to Animal Experiments
SP - 1
EP - 7
SN - 1975-9657
AB - Although fragrance-free cosmetics are increasing in recent years, fragrance is still an important element of cosmetics. The phototoxicity of fragrance used in cosmetics is tested to confirm the safety before used. Because fragrances are mixtures of various aromatic compounds and exact composition of them are not disclosed by the fragrance company, it is difficult to analyze and/or presume the cause of phototoxicity.
Therefore, it is important to use the test method having good applicability to various mixture and high reliability of results. In this study, we tried to establish an appropriate ITS approach to improve the reliability of the cosmetic fragrance toxicity test. Three animal alternatives, 3T3 neutral red uptake phototoxicity assay (OECD TG 432), Reactive oxygen species assay, Molar absorption (extinction) coefficient (MEC) measurement test were performed and coincidence rates among tests were compared. As a result, the application of the ITS approach wasn’t suitable for fragrances due to the lack of harmonization of the three test methods and different positive rates. 3T3 neutral red uptake phototoxicity assay presumed to be most suitable for the evaluation of the phototoxicity of fragrance in terms of safety. Reactive oxygen species assay and MEC measurement test were thought to be available to reconfirm the results of 3T3 neutral red uptake phototoxicity assay.
KW - Animal alternatives;3T3 NRU PT assay;ROS assay;MEC;fragrance
DO - 10.23032/jaae.2018.12.1.005
ER -
Sae-Ra Park, Sun-A Cho, Young-So Kim and Su-Sun An. (2018). Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance. Journal of Alternatives to Animal Experiments, 12(1), 1-7.
Sae-Ra Park, Sun-A Cho, Young-So Kim and Su-Sun An. 2018, "Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance", Journal of Alternatives to Animal Experiments, vol.12, no.1 pp.1-7. Available from: doi:10.23032/jaae.2018.12.1.005
Sae-Ra Park, Sun-A Cho, Young-So Kim, Su-Sun An "Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance" Journal of Alternatives to Animal Experiments 12.1 pp.1-7 (2018) : 1.
Sae-Ra Park, Sun-A Cho, Young-So Kim, Su-Sun An. Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance. 2018; 12(1), 1-7. Available from: doi:10.23032/jaae.2018.12.1.005
Sae-Ra Park, Sun-A Cho, Young-So Kim and Su-Sun An. "Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance" Journal of Alternatives to Animal Experiments 12, no.1 (2018) : 1-7.doi: 10.23032/jaae.2018.12.1.005
Sae-Ra Park; Sun-A Cho; Young-So Kim; Su-Sun An. Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance. Journal of Alternatives to Animal Experiments, 12(1), 1-7. doi: 10.23032/jaae.2018.12.1.005
Sae-Ra Park; Sun-A Cho; Young-So Kim; Su-Sun An. Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance. Journal of Alternatives to Animal Experiments. 2018; 12(1) 1-7. doi: 10.23032/jaae.2018.12.1.005
Sae-Ra Park, Sun-A Cho, Young-So Kim, Su-Sun An. Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance. 2018; 12(1), 1-7. Available from: doi:10.23032/jaae.2018.12.1.005
Sae-Ra Park, Sun-A Cho, Young-So Kim and Su-Sun An. "Evaluation of Phototoxicity through Integrated Test Strategy Approaches for fragrance" Journal of Alternatives to Animal Experiments 12, no.1 (2018) : 1-7.doi: 10.23032/jaae.2018.12.1.005
