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Establishment Study of SkinEthic TM HCE eye irritation test in Korea

  • Journal of Alternatives to Animal Experiments
  • Abbr : J Altern Anim Exp
  • 2018, 12(1), pp.1-7
  • DOI : 10.23032/jaae.2018.12.1.004
  • Publisher : Korean Society for Alternatives to Animal Experiments
  • Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
  • Received : November 28, 2018
  • Accepted : December 28, 2018
  • Published : December 29, 2018

Kyo-Hyun Park 1 Bae-Hwan Kim 1

1계명대학교 자연과학대학 공중보건학과

ABSTRACT

The objective of this study is to investigate the utility of reconstructed human corneal epithelium model(SkinEthic TM , HCE) as an alternative method to eye irritation test. We confirmed the pre-check test method for eye irritation test through condition setting of production for freeze-killed epidermis, possible direct MTT reduction with test substances, and colorant properties in test chemicals. And, we conducted eye irritation test using 8 chemicals listed in the proficiency test of the OECD test guideline 492. Summarizing the results above of assessment, it was confirmed that the SkinEthic TM HCE proves that it is so efficient and trustworthy enough to replace the eye irritation test. Taken together, the results indicated that the SkinEthic TM HCE proposed by OECD test guideline 492 is expected to become a well-established method in Korea.

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