Application of New in Vitro Test Method to Evaluate the Oral Mucosal Irritation for Toothpaste and its Ingredients (치약 및 함유 성분의 구강 점막 자극 평가를 위한 새로운 in Vitro 시험법 적용)

Sun-A Cho (조선아); Yoo-Jin Kim (김유진); Chan-Ho Kim (김찬호); Young-So Kim (김영소); Su-Sun An (안수선)

doi:10.23032/jaae.2019.13.1.004

Application of New in Vitro Test Method to Evaluate the Oral Mucosal Irritation for Toothpaste and its Ingredients

Journal of Alternatives to Animal Experiments
Abbr : J Altern Anim Exp
2019, 13(1), pp.25-38
DOI : 10.23032/jaae.2019.13.1.004
Publisher : Korean Society for Alternatives to Animal Experiments
Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
Received : February 18, 2019
Accepted : December 13, 2019
Published : December 30, 2019

Sun-A Cho ¹, Yoo-Jin Kim ¹, Chan-Ho Kim ¹, Young-So Kim ¹, Su-Sun An ¹

¹(주) 아모레퍼시픽 기술연구원

Candidate

ABSTRACT

Oral hygiene products, such as toothpastes, are essential for personal hygiene. However, these products contain substances that cause mucosal irritation but are essential to maintain functionality. Until now, there is no in vitro animal test to evaluate oral mucosal irritation for oral hygiene products and its ingredients. Moreover, oral hygiene products are classified as cosmetics in EU, thus the irritation test for these products and its ingredients are prohibited. In Korea, oral products are classified as quasi-drugs now, but there is a high possibility to change to cosmetics according to global trends. So we needed to set up the in vitro test system to evaluate oral mucosal irritation and we studied the oral mucosal irritation for toothpaste and its ingredients by agar diffusion test. In the evaluation of ingredients, the use of surfactant, tooth whitening ingredient and some functional ingredients affect the increase of cytotoxicity. In addition, when diluted to 25%, the toothpastes showed the broad range of irritation and it was appropriate to judge the level of irritation. From the results, we can set the evaluating method for toothpaste and its ingredients and internal criteria for the mucosal irritation at same time.

KEYWORDS

Toothpaste, oral mucosa, irritation, agar diffusion assay, alternative

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Application of New in Vitro Test Method to Evaluate the Oral Mucosal Irritation for Toothpaste and its Ingredients

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Citation status

* References for papers published after 2023 are currently being built.

Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Application of New in Vitro Test Method to Evaluate the Oral Mucosal Irritation for Toothpaste and its Ingredients

ABSTRACT

KEYWORDS

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