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Application of New in Vitro Test Method to Evaluate the Oral Mucosal Irritation for Toothpaste and its Ingredients

  • Journal of Alternatives to Animal Experiments
  • Abbr : J Altern Anim Exp
  • 2019, 13(1), pp.25-38
  • DOI : 10.23032/jaae.2019.13.1.004
  • Publisher : Korean Society for Alternatives to Animal Experiments
  • Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
  • Received : February 18, 2019
  • Accepted : December 13, 2019
  • Published : December 30, 2019

Sun-A Cho 1 Yoo-Jin Kim 1 Chan-Ho Kim 1 Young-So Kim 1 Su-Sun An 1

1(주) 아모레퍼시픽 기술연구원

Candidate

ABSTRACT

Oral hygiene products, such as toothpastes, are essential for personal hygiene. However, these products contain substances that cause mucosal irritation but are essential to maintain functionality. Until now, there is no in vitro animal test to evaluate oral mucosal irritation for oral hygiene products and its ingredients. Moreover, oral hygiene products are classified as cosmetics in EU, thus the irritation test for these products and its ingredients are prohibited. In Korea, oral products are classified as quasi-drugs now, but there is a high possibility to change to cosmetics according to global trends. So we needed to set up the in vitro test system to evaluate oral mucosal irritation and we studied the oral mucosal irritation for toothpaste and its ingredients by agar diffusion test. In the evaluation of ingredients, the use of surfactant, tooth whitening ingredient and some functional ingredients affect the increase of cytotoxicity. In addition, when diluted to 25%, the toothpastes showed the broad range of irritation and it was appropriate to judge the level of irritation. From the results, we can set the evaluating method for toothpaste and its ingredients and internal criteria for the mucosal irritation at same time.

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