Establishment Study on Quality and Toxicity Testing of Respiratory 3D Tissue Model (SoluAirway TM ) in Korea (호흡기 3D 조직모델(SoluAirway™)의 품질과 독성평가에 대한 국내 확립 연구)

Jeong Miho (정미호); Seol-Yeong Kim (김설영); KIMJUNWOO (김준우); Bang Injae (방인재); KIM GA EUN (김가은); Hye-Soo Kim (김혜수); Su-Hyon Lee (이수현); Kim haryong (김하룡)

doi:10.23032/jaae.2019.13.1.003

Establishment Study on Quality and Toxicity Testing of Respiratory 3D Tissue Model (SoluAirway TM ) in Korea

Journal of Alternatives to Animal Experiments
Abbr : J Altern Anim Exp
2019, 13(1), pp.17-23
DOI : 10.23032/jaae.2019.13.1.003
Publisher : Korean Society for Alternatives to Animal Experiments
Research Area : Medicine and Pharmacy > Veterinary > Basic Veterinary Medicine > Laboratory Animal Medicine
Received : December 14, 2019
Accepted : December 16, 2019
Published : December 30, 2019

Jeong Miho ¹, Seol-Yeong Kim ², KIMJUNWOO ¹, Bang Injae ¹, KIM GA EUN ³, Hye-Soo Kim ², Su-Hyon Lee ², Kim haryong ³

¹성균관대학교
²(주)바이오솔루션 부설연구소
³대구가톨릭대학교

Candidate

ABSTRACT

The concern of health and safety is growing due to increasing exposure of inhalable chemicals. However, there is no appropriate alternative testing method for assessing the hazard caused by inhalation exposure. The aim of this study is to establish respiratory 3-dimentional (3D) tissue model which can be applied for assessing the toxicity testing in Korea. First of all, respiratory 3D tissue model, SoluAirway™ was reconstituted by using human nasal mucosa. For evaluating its stability, the viability, Trans-Epithelial Electric Resistance (TEER), and histology was analyzed after delivered to 3 areas in Korea. Then, bleomycin (BLM) and polyhexamethylene guanidine phosphate (PHMG) which were known as toxic materials in pulmonary system were treated to various 3D tissue models to demonstrate applicability in evaluating chemical toxicity. The cytotoxicity and secretion of inflammatory cytokines was acquired with MTT and ELISA, respectively. The cell viability, TEER and integrity of the 3D tissue model which quality was controlled between products, had no significantly different values after delivery. The cytotoxicity of BLM and PHMG showed dose-dependently. Furthermore, the secretion of IL-8 was shown at the highest concentration of BLM and PHMG-p in SoluAirway™, not in EpiAirway™. When applied to KeraSkin™ which was a skin model, the secretion of IL-8 was declined. Taken together, these data showed that SoluAirway™ could be used as toxicity testing model for inhalable substances in Korea.

KEYWORDS

3D tissue model, SoluAirway™, Respiratory toxicity, Inhalation toxicity

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* References for papers published after 2023 are currently being built.

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Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Establishment Study on Quality and Toxicity Testing of Respiratory 3D Tissue Model (SoluAirway TM ) in Korea

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* References for papers published after 2023 are currently being built.

Journal of Alternatives to Animal Experiments 2023 KCI Impact Factor : 0.07

Establishment Study on Quality and Toxicity Testing of Respiratory 3D Tissue Model (SoluAirway TM ) in Korea

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