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@article{ART002533774},
author={박교현 and Jung-Ah Seo and Kim, Bae-Hwan},
title={The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions},
journal={Journal of Alternatives to Animal Experiments},
issn={1975-9657},
year={2019},
volume={13},
number={1},
pages={11-16},
doi={10.23032/jaae.2019.13.1.002}

TY - JOUR
AU - 박교현
AU - Jung-Ah Seo
AU - Kim, Bae-Hwan
TI - The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions
JO - Journal of Alternatives to Animal Experiments
PY - 2019
VL - 13
IS - 1
PB - Korean Society for Alternatives to Animal Experiments
SP - 11
EP - 16
SN - 1975-9657
AB - The Spectrophotometric direct peptide reactivity assay (Spectro-DPRA) is now in process for the development and validation of evaluation on skin-sensitizing chemicals. The cysteine peptides (Ac-RFAACAACOOH) and lysine peptides (Ac-RFAAKAA-COOH) is used for Spectro-DPRA. The objective of this study is to confirm the stability of cysteine and lysine peptides which are used for Spectro-DPRA through the storage conditions. We analyzed the stability of peptide using HPLC according to the conditions, such as storage temperature and storage period. The peptide powders of cysteine and lysine were stable on storage -80℃, for 6 and 12 month. And 10 mM stock peptides of cysteine and lysine were also stable on storage -70℃, for 1, 3 and 6 month. But 10 mM stock peptides of cysteine were denatured and not stable on storage -20℃, for 1 month. Taken together, peptides powder for Spectro-DPRA should be stored under -80℃, less than 12 month. And, 10 mM stock peptides should be also stored under -70℃, less than 6 month.
KW - Spectro-DPRA;cysteine;lysine;peptides;stability
DO - 10.23032/jaae.2019.13.1.002
ER -

박교현, Jung-Ah Seo and Kim, Bae-Hwan. (2019). The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions. Journal of Alternatives to Animal Experiments, 13(1), 11-16.

박교현, Jung-Ah Seo and Kim, Bae-Hwan. 2019, "The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions", Journal of Alternatives to Animal Experiments, vol.13, no.1 pp.11-16. Available from: doi:10.23032/jaae.2019.13.1.002

박교현, Jung-Ah Seo, Kim, Bae-Hwan "The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions" Journal of Alternatives to Animal Experiments 13.1 pp.11-16 (2019) : 11.

박교현, Jung-Ah Seo, Kim, Bae-Hwan. The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions. 2019; 13(1), 11-16. Available from: doi:10.23032/jaae.2019.13.1.002

박교현, Jung-Ah Seo and Kim, Bae-Hwan. "The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions" Journal of Alternatives to Animal Experiments 13, no.1 (2019) : 11-16.doi: 10.23032/jaae.2019.13.1.002

박교현; Jung-Ah Seo; Kim, Bae-Hwan. The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions. Journal of Alternatives to Animal Experiments, 13(1), 11-16. doi: 10.23032/jaae.2019.13.1.002

박교현; Jung-Ah Seo; Kim, Bae-Hwan. The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions. Journal of Alternatives to Animal Experiments. 2019; 13(1) 11-16. doi: 10.23032/jaae.2019.13.1.002

박교현, Jung-Ah Seo, Kim, Bae-Hwan. The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions. 2019; 13(1), 11-16. Available from: doi:10.23032/jaae.2019.13.1.002

박교현, Jung-Ah Seo and Kim, Bae-Hwan. "The Stability Study of Peptide for the Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA) according to Storage Conditions" Journal of Alternatives to Animal Experiments 13, no.1 (2019) : 11-16.doi: 10.23032/jaae.2019.13.1.002