@article{ART000952116},
author={Kim, Heung Joong and 정문지 and 손호현 and 박주철},
title={Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2004},
volume={17},
number={2},
pages={121-129},
doi={10.11637/aba.2004.17.2.121}
TY - JOUR
AU - Kim, Heung Joong
AU - 정문지
AU - 손호현
AU - 박주철
TI - Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines
JO - Anatomy & Biological Anthropology
PY - 2004
VL - 17
IS - 2
PB - 대한체질인류학회
SP - 121
EP - 129
SN - 2671-5651
AB - Tooth development depends on reciprocal interactions between oral epithelium and ectomesenchyme. The
ectomesenchyme-derived odontoblasts secrete several collagenous and non-collagenous proteins to form a unique
extracellular matrix. OD314 was obtained by subtractive hybridization between odontoblasts and osteoblast/pulp cells,
and differentially or predominantly expressed in odontoblasts of rat incisors compared to osteoblasts and pulp cells.
However, little is known about the function of OD314 in odontoblast differentiation. In this study, to better understand
the function of OD314, we inactivated the OD314 gene in mouse MDPC23 cells using U6 promoter-driven siRNA
method. After the characterization of mineralized nodule formation and molecular expression of MDPC23 cell,
Inactivation effects of the OD314 were evaluated by RT-PCR and western blot.
1. Mineralized nodule formation was observed after 14 days of culture in MDPC23 cells.
2. The OD314, OC and DSPP mRNA were highly expressed throughout the cultures, while the expression of ON
decreased as the MDPC23 cells differentiated.
3. The OD314 protein was weakly expressed at 7 days of culture, but increased gradually as MDPC23 cells reached
mineralization stage.
4. The inactivation of OD314 by RNA interference downregulated the expressions of OD314, DSPP, OC, and ON
mRNA and OD314 protein in MDPC23 cells.
These results suggest that OD314 may play a important role in mineralization process of odontoblast and also regulate
odontoblast-related genes such as OC, ON, and DSPP.
KW - OD314;Odontoblast;SiRNA;Mineralization;Regulation
DO - 10.11637/aba.2004.17.2.121
ER -
Kim, Heung Joong, 정문지, 손호현 and 박주철. (2004). Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines. Anatomy & Biological Anthropology, 17(2), 121-129.
Kim, Heung Joong, 정문지, 손호현 and 박주철. 2004, "Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines", Anatomy & Biological Anthropology, vol.17, no.2 pp.121-129. Available from: doi:10.11637/aba.2004.17.2.121
Kim, Heung Joong, 정문지, 손호현, 박주철 "Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines" Anatomy & Biological Anthropology 17.2 pp.121-129 (2004) : 121.
Kim, Heung Joong, 정문지, 손호현, 박주철. Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines. 2004; 17(2), 121-129. Available from: doi:10.11637/aba.2004.17.2.121
Kim, Heung Joong, 정문지, 손호현 and 박주철. "Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines" Anatomy & Biological Anthropology 17, no.2 (2004) : 121-129.doi: 10.11637/aba.2004.17.2.121
Kim, Heung Joong; 정문지; 손호현; 박주철. Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines. Anatomy & Biological Anthropology, 17(2), 121-129. doi: 10.11637/aba.2004.17.2.121
Kim, Heung Joong; 정문지; 손호현; 박주철. Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines. Anatomy & Biological Anthropology. 2004; 17(2) 121-129. doi: 10.11637/aba.2004.17.2.121
Kim, Heung Joong, 정문지, 손호현, 박주철. Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines. 2004; 17(2), 121-129. Available from: doi:10.11637/aba.2004.17.2.121
Kim, Heung Joong, 정문지, 손호현 and 박주철. "Inactivation of the OD314 gene by RNA interference in preodontoblast cell lines" Anatomy & Biological Anthropology 17, no.2 (2004) : 121-129.doi: 10.11637/aba.2004.17.2.121
