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@article{ART001081667},
author={In-Ryoung Kim and 김동진 and 이병구 and 조재범 and KIM,GYOO-CHEON and Kwak Hyun Ho and 최원철 and PARK,BONG SOO},
title={Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2007},
volume={20},
number={3},
pages={245-255},
doi={10.11637/aba.2007.20.3.245}

TY - JOUR
AU - In-Ryoung Kim
AU - 김동진
AU - 이병구
AU - 조재범
AU - KIM,GYOO-CHEON
AU - Kwak Hyun Ho
AU - 최원철
AU - PARK,BONG SOO
TI - Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells
JO - Anatomy & Biological Anthropology
PY - 2007
VL - 20
IS - 3
PB - 대한체질인류학회
SP - 245
EP - 255
SN - 2671-5651
AB - Eugenol (4-allyl-2-methoxyphenol), a major ingredient of herbs such as clove and Magnoliae Flos, is known to induce apoptosis in mast cells via p53 pathway. This study was undertaken to examine the in vivo effect of eugenol and the molecular mechanism underlying eugenol-induced apoptosis in several cancer cells with different p53 status. Effect of eugenol on mesenteric mast cells was tested using a rat anaphylaxis model. And TUNEL staining was conducted to observe the cells undergoing apoptosis. Several cancer cells were treated with eugenol, and Western blotting, immunocytochemistry, confocal microscopy and mitochondrial fractionation were conducted. Eugenol induced apoptosis in mast cells of mesentery in vivo, decreasing the density of mast cells. Although eugenol did not increase the expression level of p53, it caused the translocation of p53 into mitochondria and subsequent release of cytochrome c. Eugenol increased the level of phospho-ser 15-p53 in several cancer cells with wild type p53 but not in the cells with mutant p53 or p53 deficient cancer cell. In cancer cells with wild type p53, p53 translocated into mitochondria was phosphorylated on ser 15. In conclusion, eugenol induces apoptosis in cancer cells with wild type p53 via the translocation of phospho-ser 15- p53. Furthermore our data suggest that the anticancer effect on cancer cells with wild type p53 may be involved with the pharmacological effect of eugenol regulating apoptosis via a phospho-ser 15-p53 dependent fashion.
KW - Phospho-ser 15-p53;Eugenol;Apoptosis;Mast cell
DO - 10.11637/aba.2007.20.3.245
ER -

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철 and PARK,BONG SOO. (2007). Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells. Anatomy & Biological Anthropology, 20(3), 245-255.

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철 and PARK,BONG SOO. 2007, "Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells", Anatomy & Biological Anthropology, vol.20, no.3 pp.245-255. Available from: doi:10.11637/aba.2007.20.3.245

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철, PARK,BONG SOO "Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells" Anatomy & Biological Anthropology 20.3 pp.245-255 (2007) : 245.

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철, PARK,BONG SOO. Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells. 2007; 20(3), 245-255. Available from: doi:10.11637/aba.2007.20.3.245

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철 and PARK,BONG SOO. "Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells" Anatomy & Biological Anthropology 20, no.3 (2007) : 245-255.doi: 10.11637/aba.2007.20.3.245

In-Ryoung Kim; 김동진; 이병구; 조재범; KIM,GYOO-CHEON; Kwak Hyun Ho; 최원철; PARK,BONG SOO. Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells. Anatomy & Biological Anthropology, 20(3), 245-255. doi: 10.11637/aba.2007.20.3.245

In-Ryoung Kim; 김동진; 이병구; 조재범; KIM,GYOO-CHEON; Kwak Hyun Ho; 최원철; PARK,BONG SOO. Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells. Anatomy & Biological Anthropology. 2007; 20(3) 245-255. doi: 10.11637/aba.2007.20.3.245

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철, PARK,BONG SOO. Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells. 2007; 20(3), 245-255. Available from: doi:10.11637/aba.2007.20.3.245

In-Ryoung Kim, 김동진, 이병구, 조재범, KIM,GYOO-CHEON, Kwak Hyun Ho, 최원철 and PARK,BONG SOO. "Translocation of Phospho-ser 15-p53 in Eugenol-inducedApoptosis of in vitro Cultured Cancer Cells" Anatomy & Biological Anthropology 20, no.3 (2007) : 245-255.doi: 10.11637/aba.2007.20.3.245