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@article{ART001142692},
author={안동춘 and 전승하 and 서윤경 and 전수경 and 박현주 and 이상완 and 심정하 and 백두진},
title={The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2006},
volume={19},
number={3},
pages={165-178},
doi={10.11637/aba.2006.19.3.165}

TY - JOUR
AU - 안동춘
AU - 전승하
AU - 서윤경
AU - 전수경
AU - 박현주
AU - 이상완
AU - 심정하
AU - 백두진
TI - The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart
JO - Anatomy & Biological Anthropology
PY - 2006
VL - 19
IS - 3
PB - 대한체질인류학회
SP - 165
EP - 178
SN - 2671-5651
AB - This study was aimed to elucidate the effects of KATP activation during IPC on the PKC-ε, NF-κB and AP-1 in ischemia-reperfused rat hearts. SD male rats weighting from 300 to 350 g were split into 9 groups, such as sham control (S), IPC, 3 cycles of 5 min ischemia and 5 min reperfusion, continuous preconditioning (CP), 8 cycles of 5 min ischemia and 5 min reperfusion, KATP opening (KO) with pinacidil (1.0 mg/kg), KATP blocking with glibenclamide (1.0 mg/kg) injection, ischemia (IS), 30 min ischemia, IPC followed by IS, 8) KATP blocking and IPC followed by IS (KB+IPC+IS), IS and KATP opening (KO+IS). Heart were subjected to ligation of left descending coronary artery and reperfusion in groups of IPC, CP, IS with or without IPC. Immunohistochemistry and Western blotting for PKC-ε, NF-κB and AP-1 were performed at 3, 6, 24 hours after reperfusion or treatment. Immunoreactivities against PKC-ε antibody were observed stronger in the groups of IPC, KO, IPC+IS and KO+IS than groups of KB, IS and KB+IPC+IS. NF-κB activation and translocation were only observed in the groups of including 30 min ischemia and reperfusion. AP-1 activation and translocation were opposite to the results of PKC-ε activation. In the group of CP, KB, IS and KB+IPC+IS, reactivities of AP-1 antibody were stronger than IPC+IS, KO+IS, and weaker in the groups of S, IPC and KO. These results suggest that KATP opening with IPC or pharmacological methods may direct effect on the PKC-ε activation and that KATP blocking has effect on the AP-1 activation and translocation in the heart of ischemiareperfused of rats.
KW - Ischemic peconditioning;Rat heart;PKC-ε;NF-κB;AP-1
DO - 10.11637/aba.2006.19.3.165
ER -

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하 and 백두진. (2006). The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart. Anatomy & Biological Anthropology, 19(3), 165-178.

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하 and 백두진. 2006, "The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart", Anatomy & Biological Anthropology, vol.19, no.3 pp.165-178. Available from: doi:10.11637/aba.2006.19.3.165

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하, 백두진 "The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart" Anatomy & Biological Anthropology 19.3 pp.165-178 (2006) : 165.

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하, 백두진. The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart. 2006; 19(3), 165-178. Available from: doi:10.11637/aba.2006.19.3.165

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하 and 백두진. "The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart" Anatomy & Biological Anthropology 19, no.3 (2006) : 165-178.doi: 10.11637/aba.2006.19.3.165

안동춘; 전승하; 서윤경; 전수경; 박현주; 이상완; 심정하; 백두진. The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart. Anatomy & Biological Anthropology, 19(3), 165-178. doi: 10.11637/aba.2006.19.3.165

안동춘; 전승하; 서윤경; 전수경; 박현주; 이상완; 심정하; 백두진. The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart. Anatomy & Biological Anthropology. 2006; 19(3) 165-178. doi: 10.11637/aba.2006.19.3.165

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하, 백두진. The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart. 2006; 19(3), 165-178. Available from: doi:10.11637/aba.2006.19.3.165

안동춘, 전승하, 서윤경, 전수경, 박현주, 이상완, 심정하 and 백두진. "The Effects of Ischemic Preconditioning and KATP channel Activation on the Expression of the PKC-ε, NF-κB and AP-1 in Ischemia-reperfused Rat Heart" Anatomy & Biological Anthropology 19, no.3 (2006) : 165-178.doi: 10.11637/aba.2006.19.3.165