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@article{ART001143582},
author={Kim Min Seok and Jung Ji Yeon and 김은철 and 김현진 and Kim Won Jae and LEE EUN JOO and Sun-Hun Kim},
title={Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2005},
volume={18},
number={2},
pages={139-147},
doi={10.11637/aba.2005.18.2.139}

TY - JOUR
AU - Kim Min Seok
AU - Jung Ji Yeon
AU - 김은철
AU - 김현진
AU - Kim Won Jae
AU - LEE EUN JOO
AU - Sun-Hun Kim
TI - Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG
JO - Anatomy & Biological Anthropology
PY - 2005
VL - 18
IS - 2
PB - 대한체질인류학회
SP - 139
EP - 147
SN - 2671-5651
AB - This study is aimed to investigate the signal transduction pathway of amyloid β peptide (Aβ)-induced neuronal toxicity and the inhibitory effects of epigallocatechin gallate (EGCG), one of the major constituents of green-tea and the potent anti-oxidant, on the nerve cell damage in PC12 cells. Cellular toxicity was estimated by MTT assay and observation of morphological changes in PC12 cells. By using the methods such as measurement of Reactive Oxygen Species (ROS), western blot and RT-PCR, the underlying mechanisms and signal transduction pathway of Aβ-induced neurotoxicity and the inhibitory effects of EGCG were examined. Aβ-induced cellular toxicity was found in a dose dependent manner. This is confirmed by morphological observations of cultured cells such as findings of cell death similar to apoptosis. Aβ-induced neurotoxicity was effectively inhibited by EGCG pretreatment. Moreover, EGCG reduced ROS as same potent as the NAC (N-acetyl cystein), the ROS scavenger. Among the several process of signal transduction for cell death, a intracytoplasmic cytochrome c, the protein associated with the mitochondria-dependent pathway, was increased from 12 hours after Aβ treatment and the increased cytochrome c by Aβ was blocked by EGCG. Expression levels of Bax/Bcl-2 in relation to intracytoplasmic release of cytochrome c were examined by RT-PCR. Aβ up-regulated Bax expression but did not affect Bcl-2 expression. EGCG was found to block the effect of Aβ-induced Bax increase. From these results, it is speculated that Aβ-induced neuronal toxicity may be assumed to be affected by ROS and the mitochondria-dependent pathway of cell death as well. EGCG, besides having the role of anti-oxidant, is found to have a protective effect against Aβ-induced neurotoxicity through the inhibition of the expression of the protein associated with the mitochondria-dependent cell death pathway.
KW - Amyloid β peptide;PC12 cells;Epigallocatechine gallate;Cytotoxicity
DO - 10.11637/aba.2005.18.2.139
ER -

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO and Sun-Hun Kim. (2005). Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG. Anatomy & Biological Anthropology, 18(2), 139-147.

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO and Sun-Hun Kim. 2005, "Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG", Anatomy & Biological Anthropology, vol.18, no.2 pp.139-147. Available from: doi:10.11637/aba.2005.18.2.139

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO, Sun-Hun Kim "Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG" Anatomy & Biological Anthropology 18.2 pp.139-147 (2005) : 139.

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO, Sun-Hun Kim. Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG. 2005; 18(2), 139-147. Available from: doi:10.11637/aba.2005.18.2.139

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO and Sun-Hun Kim. "Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG" Anatomy & Biological Anthropology 18, no.2 (2005) : 139-147.doi: 10.11637/aba.2005.18.2.139

Kim Min Seok; Jung Ji Yeon; 김은철; 김현진; Kim Won Jae; LEE EUN JOO; Sun-Hun Kim. Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG. Anatomy & Biological Anthropology, 18(2), 139-147. doi: 10.11637/aba.2005.18.2.139

Kim Min Seok; Jung Ji Yeon; 김은철; 김현진; Kim Won Jae; LEE EUN JOO; Sun-Hun Kim. Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG. Anatomy & Biological Anthropology. 2005; 18(2) 139-147. doi: 10.11637/aba.2005.18.2.139

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO, Sun-Hun Kim. Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG. 2005; 18(2), 139-147. Available from: doi:10.11637/aba.2005.18.2.139

Kim Min Seok, Jung Ji Yeon, 김은철, 김현진, Kim Won Jae, LEE EUN JOO and Sun-Hun Kim. "Inhibition of Amyloid β Peptide-induced Neuronal Cytotoxicity by EGCG" Anatomy & Biological Anthropology 18, no.2 (2005) : 139-147.doi: 10.11637/aba.2005.18.2.139