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@article{ART001264900},
author={Kyung-Yong Kim and Ji Young Woo and 김기정},
title={Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={2},
pages={91-103},
doi={10.11637/aba.2008.21.2.91}

TY - JOUR
AU - Kyung-Yong Kim
AU - Ji Young Woo
AU - 김기정
TI - Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 2
PB - 대한체질인류학회
SP - 91
EP - 103
SN - 2671-5651
AB - The ancient bone DNA analysis essentially requires PCR amplification of the targeting genes of study due to the limitation of the ancient bone sample and DNA amounts. In contrast to the fresh living human DNA, it is common to face failing in amplifying the poorly preserved ancient DNA after death. Therefore, the optimized PCR methods appropriate for ancient DNA are required. However, there is no report to date that a systemic investigation of enhanced PCR amplification methods suitable for ancient samples has been conducted Approximately 500~3,300-year-old Korean and Mongolian ancient bones that are resistant to PCR were selected and an extensive number of PCR conditions were systematically investigated for the comparison of PCR success rates. For the PCR analysis, a mitochondrial DNA fragment as a multicopy DNA and a M175 Y chromosome biallelic marker DNA fragment as a single copy DNA that is the marker of the prevalent Y haplogroup (haplogroup O) in Korea were targeted. The identity of the amplified products were confirmed by DNA sequencing. Through this study, we established the optimized PCR conditions for the highly successful amplification of ancient bone DNAs. This estabilished method allowed for the successful amplification of mitochondrial DNAs from all the ancient bone samples tested and the amplification by 50% success rates in the amplification of M175 Y chromosome biallelic marker DNA but with the highest success rates. These results demonstrate that the optimized PCR condition will be useful for the promising ancient DNA analysis in the fields of molecular genetic anthropological studies.
KW - Ancient human bone;DNA;PCR;Mitochondria;Y chromosome biallelic marker
DO - 10.11637/aba.2008.21.2.91
ER -

Kyung-Yong Kim, Ji Young Woo and 김기정. (2008). Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR. Anatomy & Biological Anthropology, 21(2), 91-103.

Kyung-Yong Kim, Ji Young Woo and 김기정. 2008, "Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR", Anatomy & Biological Anthropology, vol.21, no.2 pp.91-103. Available from: doi:10.11637/aba.2008.21.2.91

Kyung-Yong Kim, Ji Young Woo, 김기정 "Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR" Anatomy & Biological Anthropology 21.2 pp.91-103 (2008) : 91.

Kyung-Yong Kim, Ji Young Woo, 김기정. Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR. 2008; 21(2), 91-103. Available from: doi:10.11637/aba.2008.21.2.91

Kyung-Yong Kim, Ji Young Woo and 김기정. "Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR" Anatomy & Biological Anthropology 21, no.2 (2008) : 91-103.doi: 10.11637/aba.2008.21.2.91

Kyung-Yong Kim; Ji Young Woo; 김기정. Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR. Anatomy & Biological Anthropology, 21(2), 91-103. doi: 10.11637/aba.2008.21.2.91

Kyung-Yong Kim; Ji Young Woo; 김기정. Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR. Anatomy & Biological Anthropology. 2008; 21(2) 91-103. doi: 10.11637/aba.2008.21.2.91

Kyung-Yong Kim, Ji Young Woo, 김기정. Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR. 2008; 21(2), 91-103. Available from: doi:10.11637/aba.2008.21.2.91

Kyung-Yong Kim, Ji Young Woo and 김기정. "Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR" Anatomy & Biological Anthropology 21, no.2 (2008) : 91-103.doi: 10.11637/aba.2008.21.2.91