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@article{ART001265207},
author={민지학 and 김민정 and In-Ryoung Kim and 이승은 and 곽현호 and 김규천 and 박혜련 and 신상훈 and Kim Chul Hoon and 정나영 and 서홍석 and 박봉수},
title={Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={2},
pages={167-180},
doi={10.11637/aba.2008.21.2.167}

TY - JOUR
AU - 민지학
AU - 김민정
AU - In-Ryoung Kim
AU - 이승은
AU - 곽현호
AU - 김규천
AU - 박혜련
AU - 신상훈
AU - Kim Chul Hoon
AU - 정나영
AU - 서홍석
AU - 박봉수
TI - Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 2
PB - 대한체질인류학회
SP - 167
EP - 180
SN - 2671-5651
AB - Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induced apoptosis in a few cancer cells in vitro. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of co-treatment with a natural product, CGM and a CDCA derivative, HS-1200 on human osteosarcoma (HOS) cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of HOS cells, MTT assay was conducted. Induction and augmentation of apoptosis were confirmed by DNA electrophoresis, Hoechst staining and DNA hypoploidy, Westen blot analysis and immunofluorescent staining were performed to study the alterations of the expression level and translocation of apoptosis-related proteins in co-treatment. Furthermore, proteasome activity and mitochondrial membrane potential (MMP) change In this study, HOS cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation whereas each single treated HOS cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 h did not induce apoptosis, the cotreatment of them induced prominently apoptosis. Therefore our data provide the possibility that combination therapy of CGM and HS-1200 could be considered as a novel therapeutic strategy for human osteosarcoma.
KW - Chios Gum Mastic;Synthetic Chenodeoxycholic Acid (CDCA) Derivatives;HS-1200;Apoptosis;Human Osteosarcoma cells
DO - 10.11637/aba.2008.21.2.167
ER -

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석 and 박봉수. (2008). Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells. Anatomy & Biological Anthropology, 21(2), 167-180.

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석 and 박봉수. 2008, "Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells", Anatomy & Biological Anthropology, vol.21, no.2 pp.167-180. Available from: doi:10.11637/aba.2008.21.2.167

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석, 박봉수 "Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells" Anatomy & Biological Anthropology 21.2 pp.167-180 (2008) : 167.

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석, 박봉수. Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells. 2008; 21(2), 167-180. Available from: doi:10.11637/aba.2008.21.2.167

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석 and 박봉수. "Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells" Anatomy & Biological Anthropology 21, no.2 (2008) : 167-180.doi: 10.11637/aba.2008.21.2.167

민지학; 김민정; In-Ryoung Kim; 이승은; 곽현호; 김규천; 박혜련; 신상훈; Kim Chul Hoon; 정나영; 서홍석; 박봉수. Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells. Anatomy & Biological Anthropology, 21(2), 167-180. doi: 10.11637/aba.2008.21.2.167

민지학; 김민정; In-Ryoung Kim; 이승은; 곽현호; 김규천; 박혜련; 신상훈; Kim Chul Hoon; 정나영; 서홍석; 박봉수. Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells. Anatomy & Biological Anthropology. 2008; 21(2) 167-180. doi: 10.11637/aba.2008.21.2.167

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석, 박봉수. Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells. 2008; 21(2), 167-180. Available from: doi:10.11637/aba.2008.21.2.167

민지학, 김민정, In-Ryoung Kim, 이승은, 곽현호, 김규천, 박혜련, 신상훈, Kim Chul Hoon, 정나영, 서홍석 and 박봉수. "Apoptotic Effect of Co-Treatment with a Natural Product,Chios Gum Mastic, and a Synthetic Chenodeoxycholic Acid Derivative,HS-1200, on Human Osteosarcoma Cells" Anatomy & Biological Anthropology 21, no.2 (2008) : 167-180.doi: 10.11637/aba.2008.21.2.167