@article{ART001302280},
author={안동춘 and Hyun-Jin Tae and 나성진 and 이석원 and 이영덕 and Hyung-Sub Kang and Sang-Youel Park and 이기창 and Yang,Hong-Hyun and In-Shik Kim},
title={Morphometric Studies in the Leydig Cell of Hemicastrated Rats},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={4},
pages={361-370},
doi={10.11637/aba.2008.21.4.361}
TY - JOUR
AU - 안동춘
AU - Hyun-Jin Tae
AU - 나성진
AU - 이석원
AU - 이영덕
AU - Hyung-Sub Kang
AU - Sang-Youel Park
AU - 이기창
AU - Yang,Hong-Hyun
AU - In-Shik Kim
TI - Morphometric Studies in the Leydig Cell of Hemicastrated Rats
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 4
PB - 대한체질인류학회
SP - 361
EP - 370
SN - 2671-5651
AB - The present study was conducted to investigate the influence of hemicastration and age at hemicastraion on the
subsequent Leydig cell morphology and function of male rats.
Sprague Dawley rats were left intact or hemicastrated at 20, 30, 40, 50, or 60 days of age (n=18 rats per group). At
100 days of age, all rats were sacrificed. Testes were fixed by whole body perfusion using a fixative containing 2.5%
glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 μm sections stained with
methylene blue, qualitative and quantitative morphological studies were performed. Testis incubations were used to
determine lutenizing hormone (LH; 100 ng/mL) stimulated testosterone secretory capacity per testis in vitro. Testosterone
levels in the incubation medium, and testosterone and LH levels in serum of these six groups of rats were
determined by radioimmunoassay.
Body and testis weights were not changed by hemicastration between experimental and control groups. Volume
density of seminiferous tubules, interstitium, and Leydig cells was not significantly affected by hemicastration.
Absolute volume of seminiferous and interstitium was significantly increased in unilaterally castrated rats at 20, 30 and
40 days of age compared to control. Significant increases in the total number of Leydig cells per testis occurred in rats
hemicastrated at 20, 30, 40 and 50 days of age compared to control. A significant increase in average volume of a
Leydig cell was noted in the hemicastrated rats at 30 and 40 days compared to intact rats of the same age but was
significantly decreased at 60 days of age. Serum testosterone levels and LH-stimulated testosterone production per
testis were significantly (P⁄0.05) increased in the hemicastrated rats at 30 and 40 days.
In summary, when rats were unilaterally castrated at 20, 30, 40, 50, and 60 days of age, those rats hemicastrated at 30
and 40 days showed compensatory hypertrophy/hypersecretion of Leydig cells when killed at 100 days of age.
Especially, these data suggested that compensatory hypertrophy/hypersecretion of Leydig cells in rats hemicastrated
around the time of puberty occurs in the remaining testis.
KW - Hemicastration;Rat;Leydig cell;Morphometry
DO - 10.11637/aba.2008.21.4.361
ER -
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun and In-Shik Kim. (2008). Morphometric Studies in the Leydig Cell of Hemicastrated Rats. Anatomy & Biological Anthropology, 21(4), 361-370.
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun and In-Shik Kim. 2008, "Morphometric Studies in the Leydig Cell of Hemicastrated Rats", Anatomy & Biological Anthropology, vol.21, no.4 pp.361-370. Available from: doi:10.11637/aba.2008.21.4.361
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun, In-Shik Kim "Morphometric Studies in the Leydig Cell of Hemicastrated Rats" Anatomy & Biological Anthropology 21.4 pp.361-370 (2008) : 361.
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun, In-Shik Kim. Morphometric Studies in the Leydig Cell of Hemicastrated Rats. 2008; 21(4), 361-370. Available from: doi:10.11637/aba.2008.21.4.361
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun and In-Shik Kim. "Morphometric Studies in the Leydig Cell of Hemicastrated Rats" Anatomy & Biological Anthropology 21, no.4 (2008) : 361-370.doi: 10.11637/aba.2008.21.4.361
안동춘; Hyun-Jin Tae; 나성진; 이석원; 이영덕; Hyung-Sub Kang; Sang-Youel Park; 이기창; Yang,Hong-Hyun; In-Shik Kim. Morphometric Studies in the Leydig Cell of Hemicastrated Rats. Anatomy & Biological Anthropology, 21(4), 361-370. doi: 10.11637/aba.2008.21.4.361
안동춘; Hyun-Jin Tae; 나성진; 이석원; 이영덕; Hyung-Sub Kang; Sang-Youel Park; 이기창; Yang,Hong-Hyun; In-Shik Kim. Morphometric Studies in the Leydig Cell of Hemicastrated Rats. Anatomy & Biological Anthropology. 2008; 21(4) 361-370. doi: 10.11637/aba.2008.21.4.361
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun, In-Shik Kim. Morphometric Studies in the Leydig Cell of Hemicastrated Rats. 2008; 21(4), 361-370. Available from: doi:10.11637/aba.2008.21.4.361
안동춘, Hyun-Jin Tae, 나성진, 이석원, 이영덕, Hyung-Sub Kang, Sang-Youel Park, 이기창, Yang,Hong-Hyun and In-Shik Kim. "Morphometric Studies in the Leydig Cell of Hemicastrated Rats" Anatomy & Biological Anthropology 21, no.4 (2008) : 361-370.doi: 10.11637/aba.2008.21.4.361
