Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33
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pISSN : 2671-5651 / eISSN : 2671-566X
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Age-Related Changes of Glutamate Transporters in the Rat Cerebellum
- Anatomy & Biological Anthropology
- Abbr : Anat Biol Anthropol
- 2008, 21(4), pp.371~380
- DOI : 10.11637/aba.2008.21.4.371
- Publisher : 대한체질인류학회
- Research Area : Medicine and Pharmacy > Anatomy
LEE EUN YOUNG 1, 최웅 1
1충북대학교
Accredited
ABSTRACT
In the present study, we examined the distribution and amount of two important glutamate transporters, GLT-1 and
GLAST in the cerebellum of young and aged rats. Sprague-Dawley rats were used at the age of three months for young
control (n=3) and 24 months for aged group (n=4). After transcardial perfusion with 4% paraformaldehyde, brain sections
were immunostained for GLT-1, and GLAST. We found that GLT-1- and GLAST-immunoreactive materials were
diffusely distributed throughout the gray matter of the cerebellum. Pre-embedding immunoelectron microscopic study
demonstrated that the two glutamate transporters in the cerebellum were restricted to glial cells with astrocytic features.
The intensity of GLT-1-immunostaining in the cerebellum appeared to be higher in aged rats than in young rats whereas
GLAST-immunostaining decreased with aging. Western blot results were also consistent with the immunohistochemical
observations. Conclusively, GLT-1 and GLAST expression in the rat cerebellum was changed with aging, i.e, increase
of GLT-1 and decrease of GLAST expression with aging, which suggests that the two glutamate transporters might be
regulated by different underlying mechanisms with aging.
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