HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing (옛사람뼈 DNA에서 HLA-DRB1 염기서열의 분석)

우지영; Kijeong Kim (김기정); 바좌라그촤 문흐체첵; Juwon Kim (김재현); 가와치멛 르학와수렝; Sohn Dong Suep (손동섭); Park Ae Ja (박애자); Kwang-Ho Lee (이광호); 김대진; Chung Yoon Hee (정윤희); Sung Su Kim (김성수); Lee Won Bok (이원복); Kyung-Yong Kim (김경용)

doi:10.11637/aba.2010.23.2.49

HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2010, 23(2), pp.49~60
DOI : 10.11637/aba.2010.23.2.49
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

우지영 ¹, Kijeong Kim ¹, 바좌라그촤 문흐체첵 ², Juwon Kim ³, 가와치멛 르학와수렝 ⁴, Sohn Dong Suep ¹, Park Ae Ja ¹, Kwang-Ho Lee ¹, 김대진 ¹, Chung Yoon Hee ¹, Sung Su Kim ¹, Lee Won Bok ¹, Kyung-Yong Kim ¹

¹중앙대학교
²중앙대학교 의과대학 미생물학교실
³동아대학교 인문과학대학 고고미술사학과
⁴중앙대학교 대학원 문화재과학과

Accredited

ABSTRACT

The analysis of ancient human DNA is increasingly used recently in the study of anthropology and human evolution. Although mitochondrial DNA and Y chromosomal DNA has commonly been the target in the field of human DNA study, HLA analysis of ancient human DNA is extremely rare. This study aimed to develop the PCR method of ancient human DNA for analyzing the sequence of HLA. Authors established a new method for HLA-DRB1 analysis by sequence-based typing. Alleles of HLA-DRB1 were analyzed and typed by sequencing with DNA of ancient human skeletons from Korea and Mongolia 3000-500years ago. The types of HLA-DRB1 were determined by comparing the sequences with those of HLA database (http://www. ebi.ac.uk/Tools/blast2/nucleotide.html). The alleles of HLA-DRB1 of ancient human DNA from Korea and Mongolia were classified by types. The frequencies of HLA-DRB1 types of Mongolia were also presented according to the geography such as West, Central, East, and North. In summary, our method was successful in the analyzing the type of HLA-DRB1 from DNA of ancient human bones. Authors anticipate that many researchers could do their research in a better way to get the genetic information for the kinship analysis between individuals or communities from ancient human bones.

KEYWORDS

Ancient human bone, DNA, HLA-DRB1, PCR

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This paper was written with support from the National Research Foundation of Korea.

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HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

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Anatomy & Biological Anthropology 2025 KCI Impact Factor : 0.29

HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

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