HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing (옛사람뼈 DNA에서 HLA-DRB1 염기서열의 분석)

우지영; Kijeong Kim (김기정); 바좌라그촤 문흐체첵; KIM JAEHYUN (김재현); 가와치멛 르학와수렝; Sohn Dong Suep (손동섭); Park Ae Ja (박애자); Kwang-Ho Lee (이광호); 김대진; Chung Yoon Hee (정윤희); Sung Su Kim (김성수); Lee Won Bok (이원복); Kyung-Yong Kim (김경용)

doi:10.11637/aba.2010.23.2.49

HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2010, 23(2), pp.49~60
DOI : 10.11637/aba.2010.23.2.49
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

우지영 ¹, Kijeong Kim ¹, 바좌라그촤 문흐체첵 ², KIM JAEHYUN ³, 가와치멛 르학와수렝 ⁴, Sohn Dong Suep ¹, Park Ae Ja ¹, Kwang-Ho Lee ¹, 김대진 ¹, Chung Yoon Hee ¹, Sung Su Kim ¹, Lee Won Bok ¹, Kyung-Yong Kim ¹

¹중앙대학교
²중앙대학교 의과대학 미생물학교실
³동아대학교 인문과학대학 고고미술사학과
⁴중앙대학교 대학원 문화재과학과

Accredited

ABSTRACT

The analysis of ancient human DNA is increasingly used recently in the study of anthropology and human evolution. Although mitochondrial DNA and Y chromosomal DNA has commonly been the target in the field of human DNA study, HLA analysis of ancient human DNA is extremely rare. This study aimed to develop the PCR method of ancient human DNA for analyzing the sequence of HLA. Authors established a new method for HLA-DRB1 analysis by sequence-based typing. Alleles of HLA-DRB1 were analyzed and typed by sequencing with DNA of ancient human skeletons from Korea and Mongolia 3000-500years ago. The types of HLA-DRB1 were determined by comparing the sequences with those of HLA database (http://www. ebi.ac.uk/Tools/blast2/nucleotide.html). The alleles of HLA-DRB1 of ancient human DNA from Korea and Mongolia were classified by types. The frequencies of HLA-DRB1 types of Mongolia were also presented according to the geography such as West, Central, East, and North. In summary, our method was successful in the analyzing the type of HLA-DRB1 from DNA of ancient human bones. Authors anticipate that many researchers could do their research in a better way to get the genetic information for the kinship analysis between individuals or communities from ancient human bones.

KEYWORDS

Ancient human bone, DNA, HLA-DRB1, PCR

Citation status

* References for papers published after 2023 are currently being built.

[journal] Alonso A / 2001 / DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples / Croatian Med J 42 : 260~266

[journal] Anzai T / 1999 / HLA genotyping of 5,000- and 6,000- year-old ancient bones in Japan / Tissue Antigens 54 : 53~58

[journal] Bar W / 2000 / Guidelines for mitochondrial DNA typing / Vox Sanguinis 79 : 121~125

[journal] Blake E / 1992 / Polymerase chain reaction (PCR) amplification and human leukocyte antigen (HLA)-DQ alpha oligonucleotide typing on biological evidence samples: casework experience / J Forensic Sci 37 : 700~726

[journal] Caillat-Zucman S / 2009 / Molecular mechanisms of HLA association with autoimmune diseases / Tissue Antigen 73 : 1~8

[journal] Calacal GC / 2005 / Fungal DNA challenge in human STR typing of bone samples / J Forensic Sci 50 : 1394~1401

[journal] Capelli C / 2003 / Ancient protocols for the crime scene? Similarities and differences between forensic genetics and ancient DNA analysis / Forensic Sci Int 131 : 59~64

[journal] Cassinotti A / 2009 / HLA and autoimmune digestive disease: a clinically oriented review for gastroenterologists / Am J Gastroenterol 104 : 195~217

[journal] Cattaneo C / 1995 / A simple method for extracting DNA from old skeletal material / Forensic Sci Int 74 : 167~174

[journal] Faerman M / 1995 / Sex identification of archaeological human remains based on amplification of the X and Y amelogenin alleles / Gene 167 : 327~332

[journal] Fischer GF / 2001 / An HLA class-II allele frequent in Eskimos and Amerindians is found in the Tyrolean Ice Man / Ann Hum Genet 65 : 363~369

[journal] Fontecchio G / 2006 / HLA-DRB genotyping of an Italian mummy from the 16th century with signs of rheumatoid arthritis / Ann Rheum Dis 65 : 1676~1677

[journal] Gilbert MT / 2005 / Assessing ancient DNA studies / Trends Ecol Evol 20 : 541~544

[journal] Haak W / 2005 / Molecular evidence of HLA-B27 in a historical case of ankylosing spondylitis / Arthritis Rheum 52 : 3318~3319

[journal] Hagelberg E / 1991 / Isolation and characterization of DNA from archaeological bone / Proc Biol Sci 244 : 45~50

[journal] Handt O / 1994 / Ancient DNA: methodological challenges / Experientia 50 : 524~529

[journal] Hänni C / 1995 / Isopropanol precipitation removes PCR inhibitors from ancient bone extracts / Nucleic Acids Res 23 : 881~882

[journal] Hansen HE / 1983 / HLA types of mummified Eskimo bodies from the 15th century / Am J Phys Anthropol 61 : 447~452

[journal] Höss M / 1996 / DNA damage and DNA sequence retrieval from ancient tissues / Nucleic Acids Res 24 : 1304~1307

[journal] Hunter P / 2006 / Ancient DNA research goes nuclear : A new technique to extract sequence data from nuclear DNA may reveal exciting new insights into evolution and phylogeny / EMBO Rep 7 : 136~139

[journal] Kalmar T / 2000 / A simple and efficient method for PCR amplifiable DNA extraction from ancient bones / Nucleic Acids Res 28 : 67~

[journal] Kemp BM / 2005 / Use of bleach to eliminate contaminating DNA from the surface of bones and teeth / Forensic Sci Int 154 : 53~61

[journal] Keyser-Tracqui C / 2005 / Methods for the study of ancient DNA / Methods Mol Biol 297 : 253~264

[journal] Kim K / 2010 / A western Eurasian male is found in 2000-year-old elite Xiongnu cemetery in Northeast Mongolia / American Journal of Physical Anthropology 20

[journal] Kim K / 2008 / Technical note: improved ancient DNA purification for PCR using ion-exchange columns / Am J Phys Anthropol 136 : 114~121

[journal] 김기정 / 2007 / Development of Ancient DNA Isolation Method for Improved PCR Amplification / 대한체질인류학회지 / 대한체질인류학회 20(4) : 267~281

[journal] 김경용 / 2008 / Establishment of PCR Reaction Condition for Highly SuccessfulAncient DNA PCR / 대한체질인류학회지 / 대한체질인류학회 21(2) : 91~103

[journal] Kolman CJ / 2000 / Ancient DNA analysis of human populations / Am J Phys Anthropol 111 : 5~23

[journal] Kumar SS / 2000 / Brief communication: discouraging prospects for ancient DNA from India / Am J Phys Anthropol 113 : 129~133

[journal] Kurosaki K / 1993 / Individual DNA identification from ancient human remains / Am J Hum Genet 53 : 638~643

[journal] Lawlor DA / 1991 / Ancient HLA genes from 7,500-year-old archaeological remains / Nature 349 : 785~788

[journal] Miao KR / 2007 / / Zhonghua Yi Xue Yi Chuan Xue Za Zhi 24 : 548~550

[journal] Mulligan CJ / 2005 / Isolation and analysis of DNA from archaeological, clinical, and natural history specimens / Methods Enzymol 395 : 87~103

[journal] O’rourke DH / 1996 / Ancient DNA: methods, progress, and perspectives / Am J Hum Biol 8 : 557~571

[journal] Pääbo S / 1989 / Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification / Proc Natl Acad Sci USA 86 : 1939~1943

[journal] Poinar HN / 1996 / Amino acid racemization and the preservation of ancient DNA / Science 272 : 846~866

[journal] Rohland N / 2007 / Comparison and optimization of ancient DNA extraction / Biotechniques 42 : 343~352

[journal] Rothschild BM / 1992 / Geographic distribution of rheumatoid arthritis in ancient North America: implication for pathogenesis / Semin Arthritis Rheum 22 : 181~187

[journal] Shiina T / 2004 / An update of the HLA genomic region, locus information and disease associations / Tissue Antigens 64 : 631~649

[journal] Yang DY / 1998 / Technical note: improved DNA extraction from ancient bones using silica-based spin columns / Am J Phys Anthropol 105 : 539~543

This paper was written with support from the National Research Foundation of Korea.

KJCKorea
Journal Central

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

HLA-DRB1 Study of DNA from Ancient Human Skeleton by Sequence-based Typing

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (0)

REFERENCES (40) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.