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@article{ART001615804},
author={김도균 and 손현진 and In-Ryoung Kim and 김규천 and 박봉수 and 곽현호},
title={Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2011},
volume={24},
number={4},
pages={203-216},
doi={10.11637/aba.2011.24.4.203}

TY - JOUR
AU - 김도균
AU - 손현진
AU - In-Ryoung Kim
AU - 김규천
AU - 박봉수
AU - 곽현호
TI - Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line
JO - Anatomy & Biological Anthropology
PY - 2011
VL - 24
IS - 4
PB - 대한체질인류학회
SP - 203
EP - 216
SN - 2671-5651
AB - Fluoride is widely used in dentistry to prevent dental caries, even though the safety of fluoride is a controversial issue. There are no known adverse effects of long-term fluoride ingestion for caries prevention, but an overdose can cause serious acute toxicity. Nevertheless it is accepted that fluoride is an important material for oral health. This study was undertaken to investigate the modulation of cell cycle-related proteins and apoptosis induction underlying mechanism by NaF treatment on G361 human melanoma cell line. The viability of G361 cells and the growth inhibition of G361 cells were assessed by MTT assay and clonogenic assay respectively. Hoechst staining, DNA electrophoresis and TUNEL staining were conducted to observe G361cells undergoing apoptosis. G361 cells were treated with NaF, and Western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, MMP activity and proteasome activity were performed. NaF treatment in G361 cells resulted in a time- and does-dependent decrease of cell viability and a does-dependent inhibition of cell growth, and induced apoptotic cell death. And tested G361 cells showed several lines of apoptotic manifestation such as nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA contents, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, a significant shift of Bax/Bcl-2 ratio, and the activation of caspase-9, caspase-7, caspase-6, caspase-3, PARP, Lamin A/C and DFF45 (ICAD). Furthermore, NaF resulted in down-regulation of the G1 cell cycle-related proteins, and up-regulation of p53. Taken collectively, our present findings demonstrate that NaF strongly inhibits cell proliferation by modulating the expression of the G1 cell cycle-related proteins and induces apoptosis via proteasome, mitochondria and caspase cascades in G361 cells.
KW - NaF;Apoptosis;Cell cycle arrest;Human melanoma cells
DO - 10.11637/aba.2011.24.4.203
ER -

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수 and 곽현호. (2011). Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line. Anatomy & Biological Anthropology, 24(4), 203-216.

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수 and 곽현호. 2011, "Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line", Anatomy & Biological Anthropology, vol.24, no.4 pp.203-216. Available from: doi:10.11637/aba.2011.24.4.203

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수, 곽현호 "Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line" Anatomy & Biological Anthropology 24.4 pp.203-216 (2011) : 203.

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수, 곽현호. Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line. 2011; 24(4), 203-216. Available from: doi:10.11637/aba.2011.24.4.203

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수 and 곽현호. "Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line" Anatomy & Biological Anthropology 24, no.4 (2011) : 203-216.doi: 10.11637/aba.2011.24.4.203

김도균; 손현진; In-Ryoung Kim; 김규천; 박봉수; 곽현호. Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line. Anatomy & Biological Anthropology, 24(4), 203-216. doi: 10.11637/aba.2011.24.4.203

김도균; 손현진; In-Ryoung Kim; 김규천; 박봉수; 곽현호. Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line. Anatomy & Biological Anthropology. 2011; 24(4) 203-216. doi: 10.11637/aba.2011.24.4.203

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수, 곽현호. Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line. 2011; 24(4), 203-216. Available from: doi:10.11637/aba.2011.24.4.203

김도균, 손현진, In-Ryoung Kim, 김규천, 박봉수 and 곽현호. "Mechanism Underlying NaF-induced Apoptosis and Cell Cycle Arrest on G361 Human Melanoma Cell Line" Anatomy & Biological Anthropology 24, no.4 (2011) : 203-216.doi: 10.11637/aba.2011.24.4.203