Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing

Lee, Jae-Ho (이재호); KIM, DAE-KWANG (김대광)

doi:10.11637/aba.2012.25.3.131

Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2012, 25(3), pp.131~136
DOI : 10.11637/aba.2012.25.3.131
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

Lee, Jae-Ho ¹, KIM, DAE-KWANG ¹

¹계명대학교

Accredited

ABSTRACT

A single nucleotide polymorphism (SNP) in the 3′-untranslated region of the KRAS oncogene (rs61764370)was recently reported to act as a genetic marker for increased risk of developing human cancers. The frequency of the variant allele is 5.8% in world population, however, there is no study in Korean population with large cases. In present study, we evaluated the KRAS let-7 lcs6 SNP in Korean population for the first time. Genotyping was performed in 178 randomized subjects. DNA was extracted and SNP was evaluated by using PCR and pyrosequencing to identify the T or G allele of the KRAS let-7 lcs6 SNP. As a result, only T allele was found in all subjects and quantitative sequencing result showed that the proportion of G allele was extremely low. This study confirmed that KRAS let-7 lcs6 have only one allele system in Korean population by sensitive and quantitative pyrosequencing for the first time. Its clinical significance should be studied further.

KEYWORDS

KRAS, Single nucleotide polymorphism (SNP), let-7 lcs6

Citation status

* References for papers published after 2023 are currently being built.

[journal] Ahmadian A / 2006 / Pyrosequencing: history, biochemistry and future / Clin Chim Acta 363 : 83~94

[confproc] Calin GA / 2004 / Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers / Proc Natl Acad Sci USA 101 : 2999~3004

[journal] Carletti MZ / 2009 / MicroRNA in the ovary and female reproductive tract / J Anim Sci 87 : 29~38

[journal] Chin LJ / 2008 / A SNP in a let-7 microRNA complementary site in the KRAS 3′ untranslated region increases non-small cell lung cancer risk / Cancer Res 68 : 8535~8540

[journal] Christensen BC / 2009 / A let-7 micro RNA-binding site polymorphism in the KRAS 3′ UTR is associated with reduced survival in oral cancers / Carcinogenesis 30 : 1003~1007

[journal] Esquela-Kerscher A / 2006 / Oncomirs: microRNAs with a role in cancer / Nat Rev Cancer 6 : 259~269

[journal] Johnson SM / 2005 / RAS is regulated by the let-7 microRNA family / Cell 120 : 635~647

[journal] Martínez-Cruz B / 2011 / In the heartland of Eurasia: the multilocus genetic landscape of Central Asian populations / Eur J Hum Genet 19 : 216~223

[journal] Mishra PJ / 2009 / MicroRNA polymorphisms: the future of pharmacogenomics, molecular epidemiology and individualized medicine / Pharmacogenomics 10 : 399~416

[journal] Nelson HH / 2010 / KRAS mutation, KRAS-LCS6 polymorphism, and non-small cell lung cancer / Lung Cancer 69 : 51~53

[journal] Paranjape T / 2011 / A 3′-untranslated region KRAS variant and triplenegative breast cancer: a case-control and genetic analysis / Lancet Oncol 12 : 377~386

[journal] Paranjape T / 2009 / MicroRNAs: tools for cancer diagnostics / Gut 58 : 1546~1554

[journal] Peng MS / 2011 / Inland post-glacial dispersal in East Asia revealed by mitochondrial haplogroup M9a’b / BMC Biol 9 : 2~

[journal] Pharoah PD / 2011 / The role of KRAS rs61764370 in invasive epithelial ovarian cancer: implications for clinical testing / Clin Cancer Res 17 : 3742~3750

[journal] Ramon D / 2003 / Pyrosequencing trade mark: a one-step method for high resolution HLA typing / J Transl Med 1(9) : 2003~

[journal] Ratner E / 2010 / A KRAS-variant in ovarian cancer acts as a genetic marker of cancer risk / Cancer Res 70 : 6509~6515

[journal] Reddy SD / 2010 / MicroRNAs and cancer therapy: the next wave or here to stay? / Cancer Biol Ther 9 : 479~482

[journal] Ronaghi M / 1998 / A sequencing method based on real-time pyrophosphate / Science 281 : 365~

[journal] Schubbert S / 2007 / Hyperactive Ras in developmental disorders and cancer / Nat Rev Cancer 7 : 295~308

[journal] Sethupathy P / 2008 / MicroRNA target site polymorphisms and human disease / Trends Genet 24 : 489~497

[journal] Smits KM / 2011 / A let-7 microRNA SNP in the KRAS 3′ UTR is prognostic in early-stage colorectal cancer / Clin Cancer Res 17 : 7723~7731

[journal] Yang H / 2008 / Evaluation of genetic variants in microRNA-related genes and risk of bladder cancer / Cancer Res 68 : 2530~2537

[journal] Yoshiura K / 2006 / A SNP in the ABCC11 gene is the determinant of human earwax type / Nat Genet 38 : 324~330

KJCKorea
Journal Central

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Evaluation of KRAS let-7 lcs6 Polymorphism in Korean Populations by Pyrosequencing

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (0)

REFERENCES (23) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.