The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation (천속단(Phlomis umbrosa Turcz)의 파골세포 분화에 미치는 영향)

백종민; 박주하; 송정훈; Park Seong Nam (박성남); 정우진; 문서영; Lee Changhoon (이창훈); 최민규; 김정중; 오재민; 장성조

doi:10.11637/aba.2013.26.3.115

The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2013, 26(3), pp.115~123
DOI : 10.11637/aba.2013.26.3.115
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

백종민 ¹, 박주하 ², 송정훈 ³, Park Seong Nam ⁴, 정우진 ², 문서영 ⁴, Lee Changhoon ⁴, 최민규 ⁴, 김정중 ⁴, 오재민 ⁴, 장성조 ⁴

¹원광대학교 의과대학 해부학교실
²원광대학교 의과대학 해부학교실 및 골격계질환연구소
³원광대학교 의과대학 성형외과학교실
⁴원광대학교

Accredited

ABSTRACT

Many bone-related diseases such as osteoporosis, rheumatoid arthritis are occurred by excessive bone resorbing activity of osteoclasts. Recently, many studies have been proceeded to find out the new therapeutic materials from natural products of plants. Phlomis umbrosa Turcz, one of the natural products of plants has been known to improve bone health. However, the precise effects and treatment mechanisms of Phlomis umbrosa Turcz about bone diseases has been unknown. So, we examined the effects of Phlomis umbrosa Turcz on expression of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) and bone resorption. Also, we investigated the treatment mechanisms of Phlomis umbrosa Turcz relating to osteoclast differentiation. Here, we showed that Phlomis umbrosa Turcz significantly suppressed RANKLinduced osteoclast differentiation and bone resportion. Furthermore, Phlomis umbrosa Turcz suppressed the activation of NF-κB in bone marrow macrophage treated RANKL and M-CSF. The mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP) in BMMs was inhibited by Phlomis umbrosa Turcz. Integrin αν, β3 relating to cell adhesion and dendritic cell-specific transmembrane protein (DC-STAMP) relating to the structure of filamentous actin (F-actin) ring and cathepsin K relating to bone resorbing activity are disrupted too. These results suggest that Phlomis umbrosa Turcz will be a good materials to treat bone diseases like osteoporosis.

KEYWORDS

Phlomis umbrosa Turcz, Osteoclast, Osteoporosis, Bone resorption, RANKL

Citation status

* References for papers published after 2023 are currently being built.

[journal] 박예수 / 2012 / Diagnosis and treatment of osteoporosis / 대한의사협회지 / 대한의사협회 55(11) : 1083~1094

[journal] Henriksen K / 2011 / Osteoclast activity and subtypes as a function of physiology and pathology-implications for future treatment of osteoporosis / Endocr Rev 23 : 31~63

[journal] David Roodman G / 2006 / Regulation of osteoclast differentiation / Ann NY Acad Sci 1068 : 100~109

[journal] Masataka A / 2007 / The molecular understanding of osteoclast differentiation / Bone 40 : 251~264

[journal] Lianping X / 2005 / Osteoclast precursors, RANKL/RANK, and immunology / Immunol Rev 208 : 19~29

[journal] Andrea DF / 2008 / Osteoclast receptors and signaling / Arch Biochem Biophys 473 : 147~160

[journal] Soysa NS / 2009 / NF-κB functions in osteoclasts / Biochem Biophys Res Commun 378 : 1~5

[journal] Huang H / 2006 / Induction of c-Fos and NFATc1 during RANKL-stimulated osteoclast differentiation is mediated by the p38 signaling pathway / Biochem Biophys Res Commun 351 : 99~105

[journal] Cheng MH / 2012 / Osteoporosis treatment in postmenopausal women with pre-existing fracture / Taiwan J Obstet Gynecol 51 : 153~166

[journal] Sundeep K / 2007 / Bisphophonates-associated osteonecrosis of the jaw:report of a task force of the american society for bone and mineral research / J Bone Miner Res 22 : 1479~1491

[book] Seo BI / Hernal Medicine / Younglimsa

[journal] Lee MS / 2009 / GM-CSF regulates fusion of mononuclear osteoclasts into bone-resorbing osteoclasts by activating the Ras/ ERK pathway / J Immunol 183 : 3390~3399

[journal] Kuhn DM / 2003 / Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism / J Clin Microbiol 41 : 506~508

[journal] Bradley EW / 2008 / Osteoclast culture and resorption assays / Methods Mol Biol 455 : 19~35

[journal] Delman PD / 2002 / Treatment of postmenopausal osteoporosis / Lancet 359 : 2018~2026

[journal] Kang MJ / 2009 / Bioavailability enhancing activities of natural compounds from medicinal plants / J Med Plant Res 13 : 1204~1211

[journal] 이명수 / 2011 / Effect of Water Extract of Saussureae Radix in RANKL-induced Osteoclast Differentiation / 동의생리병리학회지 / 대한동의병리학회 25(3) : 516~520

[journal] 오재민 / 2011 / Effect of Water Extract of Rubi Fructus in RANKL-induced Osteoclast Differentiation / 동의생리병리학회지 / 대한동의병리학회 25(4) : 669~673

[journal] 리연 / 2010 / Effect of Water Extract of Schisandra Chinensis on Osteoclast Differentiation / 동의생리병리학회지 / 대한동의병리학회 24(5) : 848~853

[journal] 김주호 / 2010 / Inhibitory Effects of Achyranthis Bidentatae Radix on Osteoclast Differentiation and Bone Resorption / 대한본초학회지 / 대한본초학회 25(1) : 65~74

[journal] Yamamoto A / 2002 / Possible involvement of IκB kinase 2 and MKK7 in osteoclastogenesis induced by receptor activator of nuclear factor kappa B ligand / J Bone Miner Res 17 : 612~621

[journal] Wada T / 2006 / RANKLRANK signaling in osteoclastogenesis and bone disease / Trends Mol Med 12 : 17~25

[journal] Kim KS / 2008 / NFATc1 induces osteoclast fusion via up-regulation of Atp6v0d2 and the dendritic cell-specific transmembrane protein (DC-STAMP) / Mol Endocrinol 22 : 176~185

[journal] Kim GS / 2005 / Association of the OSCAR promoter polymorphism with BMD in postmenopausal women / J Bone Miner Res 20 : 1342~1348

[journal] Nakamura I / 2007 / Involvement of alpha(v)beta3 integrins in osteoclast function / J Bone Miner Metab 25 : 337~344

[journal] Kukita T / 2004 / RANKL-induced DC-STAMP is essential for osteoclastogenesis / J Exp Med 200 : 941~946

[journal] Tanaka H / 2013 / Nicotine affects bone resorption and suppresses the expressions of Cathepsin K, MMP-9 and vacuolar- type(+)-ATPase d2 and organization in osteoclasts / PLoS One 8 : 59402~

KJCKorea
Journal Central

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

The Effects of Phlomis umbrosa Turcz on Osteoclast Differentiation

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (3)

REFERENCES (27) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.