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@article{ART001885786},
author={Kim Shin Young},
title={The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2014},
volume={27},
number={2},
pages={101-111},
doi={10.11637/aba.2014.27.2.101}

TY - JOUR
AU - Kim Shin Young
TI - The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury
JO - Anatomy & Biological Anthropology
PY - 2014
VL - 27
IS - 2
PB - 대한체질인류학회
SP - 101
EP - 111
SN - 2671-5651
AB - This study aimed to investigate the inflammatory changes and their main indicators according to thetime-period of postischemic reperfusion injury confirmed by analyzing changes of both pro-inflammatory andanti-inflammatory cytokines in the skeletal muscle and serum. By using 12-week-old male ICR strain mice were grouped into sham control and 8 different time-periods ofreperfusion groups (0, 0.5, 1, 2, 4, 8, 16, 24 hours). Left common iliac artery of each mice in the reperfusion groupwas devascularized by a vascular clamp for 2 hours. Once anesthesia was applied to the experimental animals,blood serum was obtained from right heart atrium on the difference time-period of reperfusion (0-, 0.5-, 1-, 2-, 4-,8-hour, respectively). Then, tissue fluid was collected in calf muscles (gastrocnemius muscle) after the mice weresacrificed by cervical dislocation. By using these serum and tissue fluids, enzyme-linked immunosorbent assay(ELISA) was used to analyze both pro-inflammatory cytokines (Eotaxin, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-5, IL-6, MCP-1, MDC, MIP-1α, RANTES, TARC, TCA-3) and anti-inflammatory cytokines (IL-4, IL-10). Consequently,there were significant differences of pro-inflammatory cytokines levels in the skeletal muscle of 0-hour reperfusion group (p⁄.05) and those in the serum of 0-, 1-, 2-, 4-, 8-, 16-hour reperfusion groups (p⁄.05). In the serum of 4-hour reperfusion group, the presence of anti-iflammatory cytokines was significant from othergroups (p⁄.05). By the comparison with the control group, furthermore, pro-inflammatory cytokines in theserum of 2-, 4-, 16-hour reperfusion group and anti-inflammatory cytokines in the serum of 4-hour reperfusiongroup were considerably different (p⁄.05). To sum up, changes of cytokine levels according to the time-period of reperfusion were considerably differentin the serum rather than the tissue fluids from the skeletal muscle. In particular, IL-6 and MCP-1 in the serumshowed higher density in 4- and 16-hour reperfusion groups so that they could be considered as the mainindicator of pro-inflammatory cytokines.
KW - Ischemia;Reperfusion;Pro-inflammatory cytokine;Anti-inflammatory cytokine
DO - 10.11637/aba.2014.27.2.101
ER -

Kim Shin Young. (2014). The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury. Anatomy & Biological Anthropology, 27(2), 101-111.

Kim Shin Young. 2014, "The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury", Anatomy & Biological Anthropology, vol.27, no.2 pp.101-111. Available from: doi:10.11637/aba.2014.27.2.101

Kim Shin Young "The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury" Anatomy & Biological Anthropology 27.2 pp.101-111 (2014) : 101.

Kim Shin Young. The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury. 2014; 27(2), 101-111. Available from: doi:10.11637/aba.2014.27.2.101

Kim Shin Young. "The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury" Anatomy & Biological Anthropology 27, no.2 (2014) : 101-111.doi: 10.11637/aba.2014.27.2.101

Kim Shin Young. The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury. Anatomy & Biological Anthropology, 27(2), 101-111. doi: 10.11637/aba.2014.27.2.101

Kim Shin Young. The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury. Anatomy & Biological Anthropology. 2014; 27(2) 101-111. doi: 10.11637/aba.2014.27.2.101

Kim Shin Young. The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury. 2014; 27(2), 101-111. Available from: doi:10.11637/aba.2014.27.2.101

Kim Shin Young. "The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury" Anatomy & Biological Anthropology 27, no.2 (2014) : 101-111.doi: 10.11637/aba.2014.27.2.101