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@article{ART001941154},
author={백종민 and 윤강휴 and 안성준 and 박선향 and 천윤희 and 김주영 and 오재민},
title={The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2014},
volume={27},
number={4},
pages={187-196},
doi={10.11637/aba.2014.27.4.187}

TY - JOUR
AU - 백종민
AU - 윤강휴
AU - 안성준
AU - 박선향
AU - 천윤희
AU - 김주영
AU - 오재민
TI - The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption
JO - Anatomy & Biological Anthropology
PY - 2014
VL - 27
IS - 4
PB - 대한체질인류학회
SP - 187
EP - 196
SN - 2671-5651
AB - Previous researches have proved that Pueraria lobata up-regulates bone mineral contents and bonemineral density in bone-loss model, ovariectomized mice and orchidectomized rats. However, the precise effectsand mechanisms of Pueraria lobata on osteoclast differentiation and bone resorbing activity of mature osteoclastsstill remains unknown. Therefore, we investigated the effect and mechanism of Pueraria lobata on receptor activatorof nuclear factor-κB ligand (RANKL) and macrophage colony stimulation factor (M-CSF)-inducedosteoclast differentiation in bone marrow macro-phages (BMMs). First of all, we treated BMMs derived frommice with various concentrations of Pueraria lobata in order to perform screening by tartrate-resistant acidphosphatase (TRAP) staining. Also, we conducted western blotting and RT-PCR for the purpose of verifying thetreatment mechanism of Pueraria lobata and lastly, we used hydroxyapatite-coated plate to evaluate the effects ofPueraria lobata on bone resorbing activity of mature osteoclasts. As a result, Pueraria lobata has inhibitoryeffect on phosphorylation of p38, Akt, c-Jun N-terminal kinase (JNK), and IκB which are essential early signalingpathway of osteoclastogenesis. Also, the inactivation of nuclear factor of activated T cells (NFAT)c1, and c-Foswhich is caused by Pueraria lobata is followed by the suppression effects of Pueraria lobata on osteoclastrelatedvarious genes, osteoclast-associated receptor (OSCAR), TRAP, Integrin β3, osteoclast stimulatorytransmembrane protein (OC-STAMP), and dendritic cell-specific transmembrane protein (DC-STAMP). Particularly, Pueraria lobata blocks the formation of pit area on hydroxyapatite-coated plate in a dose-dependentmanner as well as the mRNA expression of Cathepsin K, which is associated with bone resorbing activity. Theseresults demonstrate the molecular mechanism relating to anti-osteoclastogenesis effect of Pueraria lobata as wellas the inhibitory effect of Pueraria lobata on mature osteoclast formation and bone resorbing activity.
KW - Pueraria lobata;Osteoclast;RANKL;Bone remodeling;Osteoporosis
DO - 10.11637/aba.2014.27.4.187
ER -

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영 and 오재민. (2014). The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption. Anatomy & Biological Anthropology, 27(4), 187-196.

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영 and 오재민. 2014, "The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption", Anatomy & Biological Anthropology, vol.27, no.4 pp.187-196. Available from: doi:10.11637/aba.2014.27.4.187

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영, 오재민 "The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption" Anatomy & Biological Anthropology 27.4 pp.187-196 (2014) : 187.

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영, 오재민. The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption. 2014; 27(4), 187-196. Available from: doi:10.11637/aba.2014.27.4.187

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영 and 오재민. "The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption" Anatomy & Biological Anthropology 27, no.4 (2014) : 187-196.doi: 10.11637/aba.2014.27.4.187

백종민; 윤강휴; 안성준; 박선향; 천윤희; 김주영; 오재민. The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption. Anatomy & Biological Anthropology, 27(4), 187-196. doi: 10.11637/aba.2014.27.4.187

백종민; 윤강휴; 안성준; 박선향; 천윤희; 김주영; 오재민. The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption. Anatomy & Biological Anthropology. 2014; 27(4) 187-196. doi: 10.11637/aba.2014.27.4.187

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영, 오재민. The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption. 2014; 27(4), 187-196. Available from: doi:10.11637/aba.2014.27.4.187

백종민, 윤강휴, 안성준, 박선향, 천윤희, 김주영 and 오재민. "The Effects of Pueraria lobata on Osteoclast Differentiation and Bone Resorption" Anatomy & Biological Anthropology 27, no.4 (2014) : 187-196.doi: 10.11637/aba.2014.27.4.187