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@article{ART001941157},
author={이건호 and 이희우 and 최희정 and 민혜진 and Sun-Yong Baek and Yoon, Sik},
title={Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2014},
volume={27},
number={4},
pages={197-210},
doi={10.11637/aba.2014.27.4.197}

TY - JOUR
AU - 이건호
AU - 이희우
AU - 최희정
AU - 민혜진
AU - Sun-Yong Baek
AU - Yoon, Sik
TI - Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide
JO - Anatomy & Biological Anthropology
PY - 2014
VL - 27
IS - 4
PB - 대한체질인류학회
SP - 197
EP - 210
SN - 2671-5651
AB - The thymus is the central lymphoid organ for the development of bone marrow-derived precursorcells into mature T-cells. Understanding the molecular mechanism of thymic involution and regeneration is criticalto develop methods to normalize or improve host immunity from the decreased immune function caused bythymic involution. In this study, the regenerating thymus cDNA library was constructed in the rat from a model of thymic involutionand regeneration induced by cyclophosphamide. Expressed sequence tags (ESTs) were obtained by partialsequencing of 700 randomly selected insert-containing clones. A total of 630 ESTs were analyzed, of which 486ESTs (78%) matched to known genes and 125 ESTs (19%) matched to other ESTs (unknown genes). The 19 ESTs(3%) did not match with any known sequences. The ESTs were grouped into six main functional categories: metabolism(44%), signaling components (20%), membrane transport (7%), cytoskeleton (2%), cell division (2%) anddefense (2%). As a result of RT-PCR analysis, expression of putative gene 01, putative E2IG2 gene, musculin and osteoactivinsignificantly increased in rat thymus during regeneration. The putative gene 01 showed complete homologywith mitochondrial ribosomal protein S4 by homology search and multiple alignment of amino acid. These results provide the extensive molecular information on thymus regeneration and will be useful source toidentify various genes which may play an important role in the thymus regeneration as well as to clone novel genes. Furthermore, the availability of these data will serve as a basis for further research to understand the molecularmechanism of thymus regeneration.
KW - Expressed sequence tag (EST);Thymus;Gene expression;Cyclophosphamide;Regeneration;Rat
DO - 10.11637/aba.2014.27.4.197
ER -

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek and Yoon, Sik. (2014). Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide. Anatomy & Biological Anthropology, 27(4), 197-210.

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek and Yoon, Sik. 2014, "Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide", Anatomy & Biological Anthropology, vol.27, no.4 pp.197-210. Available from: doi:10.11637/aba.2014.27.4.197

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek, Yoon, Sik "Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide" Anatomy & Biological Anthropology 27.4 pp.197-210 (2014) : 197.

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek, Yoon, Sik. Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide. 2014; 27(4), 197-210. Available from: doi:10.11637/aba.2014.27.4.197

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek and Yoon, Sik. "Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide" Anatomy & Biological Anthropology 27, no.4 (2014) : 197-210.doi: 10.11637/aba.2014.27.4.197

이건호; 이희우; 최희정; 민혜진; Sun-Yong Baek; Yoon, Sik. Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide. Anatomy & Biological Anthropology, 27(4), 197-210. doi: 10.11637/aba.2014.27.4.197

이건호; 이희우; 최희정; 민혜진; Sun-Yong Baek; Yoon, Sik. Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide. Anatomy & Biological Anthropology. 2014; 27(4) 197-210. doi: 10.11637/aba.2014.27.4.197

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek, Yoon, Sik. Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide. 2014; 27(4), 197-210. Available from: doi:10.11637/aba.2014.27.4.197

이건호, 이희우, 최희정, 민혜진, Sun-Yong Baek and Yoon, Sik. "Expression and Characterization of Genes by Expressed Sequence Tag Analysis in the Rat Thymus during Regeneration following Acute Thymic Involution Induced by Cyclophosphamide" Anatomy & Biological Anthropology 27, no.4 (2014) : 197-210.doi: 10.11637/aba.2014.27.4.197