@article{ART001974364},
author={조혜정 and AHN KYU YOUN},
title={Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2015},
volume={28},
number={1},
pages={55-62},
doi={10.11637/aba.2015.28.1.55}
TY - JOUR
AU - 조혜정
AU - AHN KYU YOUN
TI - Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney
JO - Anatomy & Biological Anthropology
PY - 2015
VL - 28
IS - 1
PB - 대한체질인류학회
SP - 55
EP - 62
SN - 2671-5651
AB - Potassium (K) balance is regulated not only by ion channels and ion transporters, but also by variousgenes including NF-E2-related factor 2 (Nrf2). Although mRNA distribution and role of Nrf2 has been studied inhypokalemic kidney, the distribution of Nrf2 and phosphorylated-Nrf2 (p-Nrf2) proteins are not known. Thepresent study was planned to examine the alteration of expression and distribution of Nrf2 and p-Nrf2 protein inthe kidney of normal and K-depleted rats using immunohistochemistry.
In normal rat kidneys, Nrf2 was highly expressed in the proximal convoluted tubule and proximal straight tubule,moderately in cortical thick ascending limb, and weakly in cortical collecting duct, outer medullary thick ascendinglimb, and outer medullary collecting duct. In K-depleted groups, the pattern of cellular labeling of Nrf2 proteinwas identical to that of normal group, but the signal intensity was prominently increased in proximal convolutedtubule and proximal straight tubule especially in rats at K-free diet 3 weeks. In normal rat kidneys, p-Nrf2 washighly expressed in nucleus of cortical thick ascending limb, cortical collecting duct, and glomerular endothelialcell, moderately in distal convoluted tubule and outer medullary collecting duct, and weakly in proximal convolutedtubules and outer medullary thick ascending limb. In K-depleted groups, the pattern of cellular labeling ofp-Nrf2 protein was similar to that of normal group, but signal intensity was significantly increased in the nucleusof outer medullary collecting duct from of K-free diet 2 and 3 weeks groups.
These results suggest that Nrf2 and p-Nrf2 expression was gradually increased in K-depleted groups of kidney,but Nrf2 and p-Nrf2 expression patterns were not exactly matched. In addition, it is suggested that enhancedexpression of Nrf2 and p-Nrf2 in hypokalemic condition may affect the regulation of ion channels and ion transportersand subsequent intracellular signal transduction.
KW - Hypokalemia;NF-E2-related factor 2 (Nrf2);p-Nrf2;Immunohistochemistry
DO - 10.11637/aba.2015.28.1.55
ER -
조혜정 and AHN KYU YOUN. (2015). Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney. Anatomy & Biological Anthropology, 28(1), 55-62.
조혜정 and AHN KYU YOUN. 2015, "Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney", Anatomy & Biological Anthropology, vol.28, no.1 pp.55-62. Available from: doi:10.11637/aba.2015.28.1.55
조혜정, AHN KYU YOUN "Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney" Anatomy & Biological Anthropology 28.1 pp.55-62 (2015) : 55.
조혜정, AHN KYU YOUN. Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney. 2015; 28(1), 55-62. Available from: doi:10.11637/aba.2015.28.1.55
조혜정 and AHN KYU YOUN. "Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney" Anatomy & Biological Anthropology 28, no.1 (2015) : 55-62.doi: 10.11637/aba.2015.28.1.55
조혜정; AHN KYU YOUN. Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney. Anatomy & Biological Anthropology, 28(1), 55-62. doi: 10.11637/aba.2015.28.1.55
조혜정; AHN KYU YOUN. Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney. Anatomy & Biological Anthropology. 2015; 28(1) 55-62. doi: 10.11637/aba.2015.28.1.55
조혜정, AHN KYU YOUN. Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney. 2015; 28(1), 55-62. Available from: doi:10.11637/aba.2015.28.1.55
조혜정 and AHN KYU YOUN. "Alteration of Nrf2 and p-Nrf2 Protein Expression in Hypokalemic Rat Kidney" Anatomy & Biological Anthropology 28, no.1 (2015) : 55-62.doi: 10.11637/aba.2015.28.1.55
