@article{ART002032396},
author={YOO HONG IL and 정세영 and KIM SUN HUN},
title={Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2015},
volume={28},
number={3},
pages={155-165},
doi={10.11637/aba.2015.28.3.155}
TY - JOUR
AU - YOO HONG IL
AU - 정세영
AU - KIM SUN HUN
TI - Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification
JO - Anatomy & Biological Anthropology
PY - 2015
VL - 28
IS - 3
PB - 대한체질인류학회
SP - 155
EP - 165
SN - 2671-5651
AB - Bisphosphonates have been reported to have chondroprotective activities in addition to its original functions. However, mechanisms for these just began to be elucidated. Under the hypothesis that bisphosphonates may regulate expression and activities of matrix enzymes during degradation of cartilage for bone formation, we administrated an alendronate (1 mg/kg) to newborn rats subcutaneously once a day for 4, 7, and 10 days. To identify the effects of alendronate on cartilage, thickness of cartilage layer was measured by histomorphometry on the proximal epiphysis of tibia. Immunofluorescence staining and RT-PCR were performed to investigate the expressions of matrix enzymes in both in vitro and in vivo.
MTS assay revealed that at 10-3 M in concentration, alendronate significantly reduced viability of chondrocytes.
The mRNA expressions of MMP-1, MMP-9, EMMPRIN, and TIMP-3 in primary chondrocytes were decreased by the alendronate treatment. Interestingly, TIMP-1 mRNA expression was significantly increased, whereas a constitutive form, TIMP-2 was relatively unchanged by the treatment. The thickness of proliferating layer at postnatal day 7 was not significantly different, whereas thickness of hypertrophied layer was significantly thicker in the alendronate group than in the control (p<0.01). Immunofluorescence demonstrated that the expressions of MMP- 9, TIMP-2 and -3 were reduced, whereas TIMP-1 expression was increased by the alendronate administration.
These results suggest that the alendronate have chondroprotective properties by down-regulation of MMPs and up-regulation of TIMPs during endochondral ossification.
KW - Bisphosphonate;MMP;TIMP;Endochondral ossification
DO - 10.11637/aba.2015.28.3.155
ER -
YOO HONG IL, 정세영 and KIM SUN HUN. (2015). Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification. Anatomy & Biological Anthropology, 28(3), 155-165.
YOO HONG IL, 정세영 and KIM SUN HUN. 2015, "Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification", Anatomy & Biological Anthropology, vol.28, no.3 pp.155-165. Available from: doi:10.11637/aba.2015.28.3.155
YOO HONG IL, 정세영, KIM SUN HUN "Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification" Anatomy & Biological Anthropology 28.3 pp.155-165 (2015) : 155.
YOO HONG IL, 정세영, KIM SUN HUN. Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification. 2015; 28(3), 155-165. Available from: doi:10.11637/aba.2015.28.3.155
YOO HONG IL, 정세영 and KIM SUN HUN. "Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification" Anatomy & Biological Anthropology 28, no.3 (2015) : 155-165.doi: 10.11637/aba.2015.28.3.155
YOO HONG IL; 정세영; KIM SUN HUN. Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification. Anatomy & Biological Anthropology, 28(3), 155-165. doi: 10.11637/aba.2015.28.3.155
YOO HONG IL; 정세영; KIM SUN HUN. Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification. Anatomy & Biological Anthropology. 2015; 28(3) 155-165. doi: 10.11637/aba.2015.28.3.155
YOO HONG IL, 정세영, KIM SUN HUN. Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification. 2015; 28(3), 155-165. Available from: doi:10.11637/aba.2015.28.3.155
YOO HONG IL, 정세영 and KIM SUN HUN. "Effects of Bisphosphonate on the Expression of Matrix Enzymes during Endochondral Ossification" Anatomy & Biological Anthropology 28, no.3 (2015) : 155-165.doi: 10.11637/aba.2015.28.3.155
