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@article{ART002063467},
author={유보임 and 정구보},
title={Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2015},
volume={28},
number={4},
pages={223-238},
doi={10.11637/aba.2015.28.4.223}

TY - JOUR
AU - 유보임
AU - 정구보
TI - Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1
JO - Anatomy & Biological Anthropology
PY - 2015
VL - 28
IS - 4
PB - 대한체질인류학회
SP - 223
EP - 238
SN - 2671-5651
AB - Angiogenesis is the fundamental biological phenomenon in the development of vertebrates and various pathophysiological process such as cancer, inflammation and wound healing. Thrombospondin-1 is a well-known anti-angiogenic molecule which is distributed in the extracellular matrix of various tissues. The second and third type I repeats of human TSP-1 have inhibitory effects on endothelial cell migration and induce angiogenesis inhibition. However the role of the first type I repeat was not elucidated. In addition, the first type I repeat of bovine TSP-1 has CSVTCG amino acid sequence which is known to have anti-angiogenic activity. In the present study, we compared the inhibition of angiogenesis to investigate the role of the first type I repeat of the human and bovine TSP-1. Matrigel was mixed with or without TSR-1 peptides and then injected into C57BL/6J mice. We compared angiogenesis inhibition activity by hemoglobin assay, microvessel density and optical density value after 7 days. Furthermore, inhibition of angiogenesis was confirmed on CAM assay by TSR-1 peptides. For in vitro angiogenesis assay, TSR-1 peptides were treated on the proliferation, migration, and tube formation assay of HUVEC. Apoptosis effect of TSR-1 peptides was confirmed by apoptosis assay kit and flow cytometry. Bovine and human TSR-1 peptides blocked neovascularization in in vivo Matrigel plug assay and CAM assay at 10 μM. Bovine TSR-1 peptides have shown stronger angiogenesis inhibition in bFGF-induced angiogenesis than human TSR-1 and CSVTCG peptides. However, all of TSR-1 peptides inhibit migration and tube formation of HUVEC in in vitro. Furthermore, these peptides also induced apoptosis of HUVEC. These results suggest that TSR-1 peptides of bovine and human TSP-1 have angiogenesis inhibition activity.
KW - Angiogenesis;Thrombospondin-1;TSR-1;Bovine TSP-1;Endothelial cell
DO - 10.11637/aba.2015.28.4.223
ER -

유보임 and 정구보. (2015). Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1. Anatomy & Biological Anthropology, 28(4), 223-238.

유보임 and 정구보. 2015, "Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1", Anatomy & Biological Anthropology, vol.28, no.4 pp.223-238. Available from: doi:10.11637/aba.2015.28.4.223

유보임, 정구보 "Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1" Anatomy & Biological Anthropology 28.4 pp.223-238 (2015) : 223.

유보임, 정구보. Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1. 2015; 28(4), 223-238. Available from: doi:10.11637/aba.2015.28.4.223

유보임 and 정구보. "Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1" Anatomy & Biological Anthropology 28, no.4 (2015) : 223-238.doi: 10.11637/aba.2015.28.4.223

유보임; 정구보. Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1. Anatomy & Biological Anthropology, 28(4), 223-238. doi: 10.11637/aba.2015.28.4.223

유보임; 정구보. Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1. Anatomy & Biological Anthropology. 2015; 28(4) 223-238. doi: 10.11637/aba.2015.28.4.223

유보임, 정구보. Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1. 2015; 28(4), 223-238. Available from: doi:10.11637/aba.2015.28.4.223

유보임 and 정구보. "Inhibition of Angiogenesis by the First Type I Repeat Peptides of Thrombospondin-1" Anatomy & Biological Anthropology 28, no.4 (2015) : 223-238.doi: 10.11637/aba.2015.28.4.223