Effect of MITF-M and MITF-A Overexpression on the Dendrtic Formation in Melanocytes (MITF (microphthalmia-associated transcription factor)가 멜라닌세포의 가지돌기 형성에 미치는 영향)

조인숙; 손경철; Kim Chang Deok (김창덕); Lee， Young Ho (이영호)

doi:10.11637/aba.2016.29.1.27

Effect of MITF-M and MITF-A Overexpression on the Dendrtic Formation in Melanocytes

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2016, 29(1), pp.27~34
DOI : 10.11637/aba.2016.29.1.27
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

조인숙 ¹, 손경철 ², Kim Chang Deok ², Lee， Young Ho ²

¹충남대학교 의과대학 해부학교실
²충남대학교

Accredited

ABSTRACT

The microphthalmia-associated transcription factor (MITF), has been described as the master regulator of the basic helix-loop-helix leucine zipper family, involves melanogenesis in melanocytes. MITF consists of at least six isoforms, called MITF-M, MITF-A, MITF-B, MITF-C, MITF-H, and MITF-J. Previously, we found that not only MITF-M is expressed in the human hair follicle, but also MITF-A, MITF-C, MITF-H, and MITF-J isoforms are expressed in the skin. The aim of this study was to conform the MITF isoforms expressed in human skin, and investigate novel role of MITF isoforms in the melanocytes. Expression of MITF-M and MITF-A was found in primary melanoctyes and the melanoma cell lines. Interestingly, when MITF-M and MITF-A were overexpressed in the SK-MEL-24 melanoma cells by adenoviral transfection, length of the dendrites, serves as the principal conduit for melanosomes transfer, was significantly increased in the MITF-M overexpressed cells compared with the control group, and number of the dendtrites was significantly increased in the MITF-A overexpressed cells. A signal molecule involve in actin polymerization during dendrite formation, Rac1, was increased in the SK-MEL-24 melanoma cells treated with adenoviral MITF-M and MITF-A vectors. These results suggest that MITF-M and MITF-A induce dendrite formation via Rac1 signaling in the melanocytes.

KEYWORDS

MITF, Melanocytes, Dendrites

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This paper was written with support from the National Research Foundation of Korea.

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Effect of MITF-M and MITF-A Overexpression on the Dendrtic Formation in Melanocytes

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Effect of MITF-M and MITF-A Overexpression on the Dendrtic Formation in Melanocytes

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