@article{ART002386199},
author={Kim， Joo Young and KIM, DAE-KWANG},
title={Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2018},
volume={31},
number={3},
pages={77-82},
doi={10.11637/aba.2018.31.3.77}
TY - JOUR
AU - Kim， Joo Young
AU - KIM, DAE-KWANG
TI - Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)
JO - Anatomy & Biological Anthropology
PY - 2018
VL - 31
IS - 3
PB - 대한체질인류학회
SP - 77
EP - 82
SN - 2671-5651
AB - Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis is a kind of sensitive mutation detection method that has been usually used in field of medical genetics. A single DNA strand with a mutation or nucleotide polymorphism has a different conformation from its wild-type counterpart, and these conformational differences result in different electrophoretic mobility. In previous study of mitochondrial microsatellite instability in 50 uterine leiomyomas, PCR-SSCP showed 4 types of band mobility at (CA)n of the mitochondrial D-loop. In type 1 and 4, positions of the lower single stand of both were same but those of upper strand were different. In sequencing analysis, repeat number of (CA)n in type 1 was 4, 5 in type 2, 6 in type 3, and 4 in type 4, respectively. Without using expensive sequencing analysis, PCR-SSCP method can be used to detect the repeat number of (CA)n in mitochondrial D-loop.
KW - PCR-SSCP;Mitochondrial D-loop;(CA)n repeat number
DO - 10.11637/aba.2018.31.3.77
ER -
Kim， Joo Young and KIM, DAE-KWANG. (2018). Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP). Anatomy & Biological Anthropology, 31(3), 77-82.
Kim， Joo Young and KIM, DAE-KWANG. 2018, "Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)", Anatomy & Biological Anthropology, vol.31, no.3 pp.77-82. Available from: doi:10.11637/aba.2018.31.3.77
Kim， Joo Young, KIM, DAE-KWANG "Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)" Anatomy & Biological Anthropology 31.3 pp.77-82 (2018) : 77.
Kim， Joo Young, KIM, DAE-KWANG. Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP). 2018; 31(3), 77-82. Available from: doi:10.11637/aba.2018.31.3.77
Kim， Joo Young and KIM, DAE-KWANG. "Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)" Anatomy & Biological Anthropology 31, no.3 (2018) : 77-82.doi: 10.11637/aba.2018.31.3.77
Kim， Joo Young; KIM, DAE-KWANG. Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP). Anatomy & Biological Anthropology, 31(3), 77-82. doi: 10.11637/aba.2018.31.3.77
Kim， Joo Young; KIM, DAE-KWANG. Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP). Anatomy & Biological Anthropology. 2018; 31(3) 77-82. doi: 10.11637/aba.2018.31.3.77
Kim， Joo Young, KIM, DAE-KWANG. Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP). 2018; 31(3), 77-82. Available from: doi:10.11637/aba.2018.31.3.77
Kim， Joo Young and KIM, DAE-KWANG. "Determination of Repeat Numbers of (CA)n in Mitochondrial D-loop using Polymerase Chain Reaction-single Strand Conformational Polymorphism (PCR-SSCP)" Anatomy & Biological Anthropology 31, no.3 (2018) : 77-82.doi: 10.11637/aba.2018.31.3.77
