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@article{ART002852169},
author={Nanda Maya Mali and Jung Min Park and KIM GIL HYUN and Choi Dong-Hwa and Raul Ramos and JongHyuk Lee and Eu Jeong Ku and JI WON OH},
title={Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2022},
volume={35},
number={2},
pages={57-66},
doi={10.11637/aba.2022.35.2.57}

TY - JOUR
AU - Nanda Maya Mali
AU - Jung Min Park
AU - KIM GIL HYUN
AU - Choi Dong-Hwa
AU - Raul Ramos
AU - JongHyuk Lee
AU - Eu Jeong Ku
AU - JI WON OH
TI - Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas
JO - Anatomy & Biological Anthropology
PY - 2022
VL - 35
IS - 2
PB - 대한체질인류학회
SP - 57
EP - 66
SN - 2671-5651
AB - Optical imaging of the vascular system is necessary for structural, functional, developmental, and pathological biology. Recent developments in optical tissue-clearing methods have significantly advanced largescale tissue researches by integrating a three-dimensional (3D) visualization of topographical vasculature, so we aimed to enhance the vascular structure of the murine pancreas which are highly vascularized structure. In this study, the adult C57/BL6 murine pancreatic tissue was cleared after lectin staining, resulting in the reconstructed structures for 3D imaging within a short period without damaging. Enhancing the visualization processes using light-sheet fluorescence microscopy (LSFM) together with 3D reconstruction imaging tool, we studied in depth of the entire vascular system, which further enabled to reconstruct 3D structure of the vasculature in pancreas and islets. We successfully implemented the normal murine pancreatic tissues including exocrine and endocrine cells and vascular structures, reflecting pancreatic β-cell function. The size of islets of each pancreatic lobe were not different but the number of islets in each lobe was variable in our quantification. In conclusion, this deep tissue clearing technique for 3D imaging could be applied in future studies to further understand the topological interactions of various cells and blood vessels within intact organs and tissues.
KW - Light-sheet fluorescence microscopy;Pancreas;Three-dimensional;Tissue clearing;Vascular system
DO - 10.11637/aba.2022.35.2.57
ER -

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku and JI WON OH. (2022). Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas. Anatomy & Biological Anthropology, 35(2), 57-66.

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku and JI WON OH. 2022, "Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas", Anatomy & Biological Anthropology, vol.35, no.2 pp.57-66. Available from: doi:10.11637/aba.2022.35.2.57

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku, JI WON OH "Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas" Anatomy & Biological Anthropology 35.2 pp.57-66 (2022) : 57.

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku, JI WON OH. Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas. 2022; 35(2), 57-66. Available from: doi:10.11637/aba.2022.35.2.57

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku and JI WON OH. "Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas" Anatomy & Biological Anthropology 35, no.2 (2022) : 57-66.doi: 10.11637/aba.2022.35.2.57

Nanda Maya Mali; Jung Min Park; KIM GIL HYUN; Choi Dong-Hwa; Raul Ramos; JongHyuk Lee; Eu Jeong Ku; JI WON OH. Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas. Anatomy & Biological Anthropology, 35(2), 57-66. doi: 10.11637/aba.2022.35.2.57

Nanda Maya Mali; Jung Min Park; KIM GIL HYUN; Choi Dong-Hwa; Raul Ramos; JongHyuk Lee; Eu Jeong Ku; JI WON OH. Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas. Anatomy & Biological Anthropology. 2022; 35(2) 57-66. doi: 10.11637/aba.2022.35.2.57

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku, JI WON OH. Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas. 2022; 35(2), 57-66. Available from: doi:10.11637/aba.2022.35.2.57

Nanda Maya Mali, Jung Min Park, KIM GIL HYUN, Choi Dong-Hwa, Raul Ramos, JongHyuk Lee, Eu Jeong Ku and JI WON OH. "Deep Tissue Clearing for Three-dimensional Imaging Analysis of Murine Pancreas" Anatomy & Biological Anthropology 35, no.2 (2022) : 57-66.doi: 10.11637/aba.2022.35.2.57