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@article{ART002063482},
author={Yung Hyun Choi},
title={Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts},
journal={Journal of Korean Medicine for Obesity Research},
issn={1976-9334},
year={2015},
volume={15},
number={2},
pages={93-103}

TY - JOUR
AU - Yung Hyun Choi
TI - Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts
JO - Journal of Korean Medicine for Obesity Research
PY - 2015
VL - 15
IS - 2
PB - The Society of Korean Medicine for Obesity Research
SP - 93
EP - 103
SN - 1976-9334
AB - Objectives: It was investigated the cytoprotective efficacies of isorhamnetin, a flavonoidoriginally derived from Hippophae rhamnoides L., against oxidative stress-induced apoptosis inC2C12 myoblasts. Methods: The effects of isorhamnetin on cell growth, apoptosis and reactive oxygen species(ROS) generation were evaluated by trypan blue dye exclusion assay, 4',6-diamidino-2-phenylindolestaining and flow cytometry. The levels of apoptosis-regulatory and nuclear factorerythroid 2-related factor 2 (Nrf2) signaling pathway-related proteins, and caspase activities(caspase-3 and -9) were determined by Western blot analysis and colorimetric assay, respectively. Results: Our results revealed that treatment with isorhamnetin prior to hydrogen peroxide (H2O2)exposure significantly increased the C2C12 cell viability and, indicating that the exposure ofC2C12 cells to isorhamnetin conferred a protective effect against oxidative stress. Isorhamnetinalso effectively attenuated H2O2-induced apoptosis and ROS generation, which was associatedwith the restoration of the upregulation of Bax and downregulation of Bcl-2 induced by H2O2. Inaddition, H2O2 enhanced the activation of caspase-9 and -3, and degradation of poly (ADPribose)-polymerase, a typical substrate protein of activated caspase-3; however, these eventswere almost totally reversed by pretreatment with isorhamnetin. Moreover, isorhamnetinincreased the levels of heme oxygenase-1, a potent antioxidant enzyme, associated with theinduction of Nrf2. Conclusions: Our data indicated that isorhamnetin may potentially serve as an agent for thetreatment and prevention of muscle disorders caused by oxidative stress.
KW - 3-methylquercetin;Oxidative stress;Reactive oxygen species;Apoptosis;Nuclear factor erythroid 2-related factor 2/heme oxygenase-1
DO -
UR -
ER -

Yung Hyun Choi. (2015). Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts. Journal of Korean Medicine for Obesity Research, 15(2), 93-103.

Yung Hyun Choi. 2015, "Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts", Journal of Korean Medicine for Obesity Research, vol.15, no.2 pp.93-103.

Yung Hyun Choi "Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts" Journal of Korean Medicine for Obesity Research 15.2 pp.93-103 (2015) : 93.

Yung Hyun Choi. Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts. 2015; 15(2), 93-103.

Yung Hyun Choi. "Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts" Journal of Korean Medicine for Obesity Research 15, no.2 (2015) : 93-103.

Yung Hyun Choi. Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts. Journal of Korean Medicine for Obesity Research, 15(2), 93-103.

Yung Hyun Choi. Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts. Journal of Korean Medicine for Obesity Research. 2015; 15(2) 93-103.

Yung Hyun Choi. Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts. 2015; 15(2), 93-103.

Yung Hyun Choi. "Protective Effects of Isorhamnetin against Hydrogen Peroxide-Induced Apoptosis in C2C12 Murine Myoblasts" Journal of Korean Medicine for Obesity Research 15, no.2 (2015) : 93-103.