The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA (코엔자임 Q10 처리에 따른 TEGDMA에 의해 유발된 치아세포 사멸 억제 효과)

Ahreum Lee (이아름); 박소영; kyung Hee Lee (이경희)

doi:10.13065/jksdh.2014.14.05.775

The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA

Journal of Korean society of Dental Hygiene
Abbr : J Korean Soc Dent Hyg
2014, 14(5), pp.775-781
DOI : 10.13065/jksdh.2014.14.05.775
Publisher : Korean Society of Dental Hygiene
Research Area : Medicine and Pharmacy > Dentistry

Ahreum Lee ¹, 박소영 ¹, kyung Hee Lee ²

¹동서대학교 치위생학과
²동서대학교

Accredited

ABSTRACT

Objectives : The purpose of the study is to investigate the protective effect of coenzyme Q10 on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme Q10. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme Q10 showed cell proliferation from 5 to 500 µM by WST-1 assay. Pre-treatment coenzyme Q10 showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme Q10 treatment group(group 2) in comparison with coenyme Q10 pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme Q10 showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.

KEYWORDS

antioxidant, cell death, coenzyme Q10, odontoblast, triethylene glycol dimethacrylate(TEGDMA)

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This paper was written with support from the National Research Foundation of Korea.

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Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA

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* References for papers published after 2023 are currently being built.

Journal of Korean society of Dental Hygiene 2023 KCI Impact Factor : 0.8

The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA

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