@article{ART003075885},
author={심선주 and 김지혜 and 신혜선},
title={Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods},
journal={Journal of Korean society of Dental Hygiene},
issn={2287-1705},
year={2024},
volume={24},
number={2},
pages={131-139},
doi={10.13065/jksdh.20240014}
TY - JOUR
AU - 심선주
AU - 김지혜
AU - 신혜선
TI - Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods
JO - Journal of Korean society of Dental Hygiene
PY - 2024
VL - 24
IS - 2
PB - Korean Society of Dental Hygiene
SP - 131
EP - 139
SN - 2287-1705
AB - Objectives: Oral bacterial samples included subgingival, supragingival, and saliva plaques. As the diversity and number of microorganisms deffer depending on the area of the oral cavity and the method used, an appropriate and reliable collection method is important. The present study investigated oral bacterial sampling methods. Methods: Supragingival dental plaque was collected from the buccal and lingual tooth surfaces of study participants using sterilized cotton swabs. Plaques were collected from the subgingival area using a sterilized curette. Bacterial genomic DNA was extracted using MagNA Pure 96 DNA and Viral NA low-volume kits. Real-time polymerase chain reaction (PCR) was performed using the PowerCheck™ Periodontitis Pathogens Multiplex Real-time PCR kit. Results: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Fusobacterium nucleatum of the orange complex were not observed in the subgingival biofilms of all study participants. For Porphyromonas. gingivalis, a significant correlation was observed between supragingival, subgingival, and total tooth surface biofilms. Compared to the supragingival and subgingival biofilmss, total tooth surface biofilm exhibited the highest bacterial count when the inswabbing method was used. Conclusions: Based on these findings, the supragingival swab method is recommended for oral bacterial research.
KW - Biofilm;Dental plaque;Real-time polymerase reaction;Specimen handling
DO - 10.13065/jksdh.20240014
ER -
심선주, 김지혜 and 신혜선. (2024). Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods. Journal of Korean society of Dental Hygiene, 24(2), 131-139.
심선주, 김지혜 and 신혜선. 2024, "Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods", Journal of Korean society of Dental Hygiene, vol.24, no.2 pp.131-139. Available from: doi:10.13065/jksdh.20240014
심선주, 김지혜, 신혜선 "Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods" Journal of Korean society of Dental Hygiene 24.2 pp.131-139 (2024) : 131.
심선주, 김지혜, 신혜선. Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods. 2024; 24(2), 131-139. Available from: doi:10.13065/jksdh.20240014
심선주, 김지혜 and 신혜선. "Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods" Journal of Korean society of Dental Hygiene 24, no.2 (2024) : 131-139.doi: 10.13065/jksdh.20240014
심선주; 김지혜; 신혜선. Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods. Journal of Korean society of Dental Hygiene, 24(2), 131-139. doi: 10.13065/jksdh.20240014
심선주; 김지혜; 신혜선. Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods. Journal of Korean society of Dental Hygiene. 2024; 24(2) 131-139. doi: 10.13065/jksdh.20240014
심선주, 김지혜, 신혜선. Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods. 2024; 24(2), 131-139. Available from: doi:10.13065/jksdh.20240014
심선주, 김지혜 and 신혜선. "Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods" Journal of Korean society of Dental Hygiene 24, no.2 (2024) : 131-139.doi: 10.13065/jksdh.20240014
