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Preliminary study on the diversity and quantity analysis of oral bacteria according to the sampling methods

  • Journal of Korean society of Dental Hygiene
  • Abbr : J Korean Soc Dent Hyg
  • 2024, 24(2), pp.131-139
  • DOI : 10.13065/jksdh.20240014
  • Publisher : Korean Society of Dental Hygiene
  • Research Area : Medicine and Pharmacy > Dentistry
  • Received : March 13, 2024
  • Accepted : April 12, 2024
  • Published : April 30, 2024

심선주 1 김지혜 1 신혜선 2

1백석대학교 치위생학과
2동남보건대학교 치위생학과

Accredited

ABSTRACT

Objectives: Oral bacterial samples included subgingival, supragingival, and saliva plaques. As the diversity and number of microorganisms deffer depending on the area of the oral cavity and the method used, an appropriate and reliable collection method is important. The present study investigated oral bacterial sampling methods. Methods: Supragingival dental plaque was collected from the buccal and lingual tooth surfaces of study participants using sterilized cotton swabs. Plaques were collected from the subgingival area using a sterilized curette. Bacterial genomic DNA was extracted using MagNA Pure 96 DNA and Viral NA low-volume kits. Real-time polymerase chain reaction (PCR) was performed using the PowerCheck™ Periodontitis Pathogens Multiplex Real-time PCR kit. Results: Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Fusobacterium nucleatum of the orange complex were not observed in the subgingival biofilms of all study participants. For Porphyromonas. gingivalis, a significant correlation was observed between supragingival, subgingival, and total tooth surface biofilms. Compared to the supragingival and subgingival biofilmss, total tooth surface biofilm exhibited the highest bacterial count when the inswabbing method was used. Conclusions: Based on these findings, the supragingival swab method is recommended for oral bacterial research.

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