The purpose of this study is to investigate the dyeability of silk fabrics with Humulus japonicus (Hwansam)extract which is a kind of weeds that grows wild in the suburbs. In the research, the general extract method wasconducted with the extract from fresh herb of root-removed Humulus japonicus while we investigated the optimal dyeingconditions and dyeability depending on the changes of the kind of mordants, methods and temperature. The functionalproperties such as antibacterial activities, deodorization and UV protection were also reviewed. The ideal output, as aresult, was shown at 100% of dye concentration, 90℃ of dyeing temperature and 40 minutes of dyeing time while interms of dye uptake depending on the kind of mordants and methods, it was found that among four mordants of SnCl2,AlK(SO4)2, CuSO4, and FeSO4 post-mordanting with FeSO4 showed the best result. Color fastness to washing, drycleaning and rubbing was found strong at grade 4-5 and the grade to light was as good as 3 while to perspiration wasgood at 4-5 as well. In aspects of functional properties, it showed excellent results of 99% deodorization rate, 98% UVprotection rate and 99.9% bacterial reduction against Staphylococcus aureus.