@article{ART001727161},
author={이장호 and Jong-Chun Lee and MYUNGJIN KIM and 한아름 and 이유진 and 바데 라빈드라 and 김민성},
title={Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank},
journal={Journal of Environmental Impact Assessment},
issn={1225-7184},
year={2012},
volume={21},
number={6},
pages={823-839}
TY - JOUR
AU - 이장호
AU - Jong-Chun Lee
AU - MYUNGJIN KIM
AU - 한아름
AU - 이유진
AU - 바데 라빈드라
AU - 김민성
TI - Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank
JO - Journal of Environmental Impact Assessment
PY - 2012
VL - 21
IS - 6
PB - Korean Society Of Environmental Impact Assessment
SP - 823
EP - 839
SN - 1225-7184
AB - Environmental Specimen Banks (ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystem based on the retrospective analysis of the representative samples collected regularly and stored in cryogenic condition. In Korea, National Environmental Specimen Bank (NESB) was established in 2009 and the standard operating procedures (SOPs) for sampling, and cryogenic milling and storage had been prepared during 2007-2010. Since then, the tentative SOPs for the seven kinds of specimens (shoots of Red Pine (Pinus densiflora) and Korean Pine (Pinus koraiensis), leaves of Mongolian Oak (Quercus mongolica) and Zelkova Tree (Zelkova serrata), eggs of Feral Pigeon (Columba livia var. domestica), muscles and organs of Common Carp (Cyprinus carpio), and Freshwater Bivalve (Unio (Nodularia) douglasiae)) have been put to test in the field and laboratory as well against the practicality and feasibility. The SOPs were improved by reflecting the findings from the research and the following discussion regarding the selection of specimen (Feral Pigeon suffering from a control management), sample size (a problem of decreasing number of sampling trees related to increasing sampling time) and period (a problem related to a bud growth), and sampling methods etc.. In addition, barcoding system for the management of the specimen information, and monitoring system of the cryogenic storage to regulate the optimum temperature and the liquid nitrogen level were also developed for the efficient and effective control of the samples. Lastly, the safety guide and emergency protocol were augmented to guarantee a safe work environment with the cryogenic facility. These improvements of the SOPs are expected to contribute to more stable operation of the NESB.
KW - Environmental Samples;Standard Operating Procedures;Cryogenic Storage
DO -
UR -
ER -
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라 and 김민성. (2012). Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank. Journal of Environmental Impact Assessment, 21(6), 823-839.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라 and 김민성. 2012, "Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank", Journal of Environmental Impact Assessment, vol.21, no.6 pp.823-839.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라, 김민성 "Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank" Journal of Environmental Impact Assessment 21.6 pp.823-839 (2012) : 823.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라, 김민성. Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank. 2012; 21(6), 823-839.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라 and 김민성. "Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank" Journal of Environmental Impact Assessment 21, no.6 (2012) : 823-839.
이장호; Jong-Chun Lee; MYUNGJIN KIM; 한아름; 이유진; 바데 라빈드라; 김민성. Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank. Journal of Environmental Impact Assessment, 21(6), 823-839.
이장호; Jong-Chun Lee; MYUNGJIN KIM; 한아름; 이유진; 바데 라빈드라; 김민성. Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank. Journal of Environmental Impact Assessment. 2012; 21(6) 823-839.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라, 김민성. Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank. 2012; 21(6), 823-839.
이장호, Jong-Chun Lee, MYUNGJIN KIM, 한아름, 이유진, 바데 라빈드라 and 김민성. "Sampling and Cryogenic Pulverization and Storage of Environmental Samples and Improvement of Operating Procedures in National Environmental Specimen Bank" Journal of Environmental Impact Assessment 21, no.6 (2012) : 823-839.