Purpose: In this descriptive study, we determined the perceptions of patient safety culture among health professionals, including nurses, doctors, medical technicians, and hospital administrative staff. Methods: A questionnaire survey was used to collect data; specifically, we distributed questionnaires to 317 health professionals working for P National University Hospital in B Metropolitan city and P National University Hospital in Y City. The mean and standard deviations of the degree of awareness of patient safety culture were analyzed using t-tests and ANOVAs. Post-hoc comparisons were made with Scheffé's test. Results: The mean score for perception of patient safety culture among study subjects was 3.43 out of 5, indicating that it was in the average range. When analyzing by position, nurses, medical technicians, and administrative staff tended to have better perceptions of patient safety culture than did doctors (F=22.13, p<.001). Furthermore, perceptions among health professionals appeared to vary by their age, education level, years of working in the current hospital, weekly work hours, work unit, attendance of safety education in the past year, number of patient safety education sessions attended in the past year, and awareness of the importance of accurate reporting procedures for safety accidents. Conculsion: As this study confirms, medical professionals’ prior experience with patient safety education, number of training sessions attended, and awareness of the reporting procedures for safety accidents influence their perceptions of the patient safety culture. Therefore, patient safety education should be promoted in line with the characteristics of each occupation. Additionally, it is recommended that cultures encouraging health professionals to actively attend the necessary training be established in hospitals.