As the necessity of producing 1:5,000 Environmental Conservation Value Assessment Map (ECVAM) has increased especially in fields of national land and environmental planning, 1:5,000 ECVAM has been established starting from Boryeong city in 2013. Among the 8 environmental-ecological assessment items consisting ECVAM, ‘Naturalness’ and ‘Stability’ are based on the Biotope map created by each local governments. However, only 88 out of 230 local government have constructed the Biotope map, urging an alternative assessment approach for the rest 142 local governments to assess ‘Naturalness’ and ‘Stability’ of 1:5,000 ECVAM. Therefore in this study, we examined the applicability of 1:5,000 precise forest type map by comparing results using 1:25,000 5th forest type map, 1:5,000 actual vegetation map of Biotope Map, and 1:5,000 Precise Forest Type Map as an alternative layer in assessing ‘Naturalness’ and ‘Stability’ of environmental-ecological assessment. As a result of comparing the result of ‘Naturalness’ and ‘Stability’ using 1:5,000 actual vegetation map and 1:5,000 precise forest type map, the area remaining the same assessment grade occupied 44.99% and 58.77%, respectively. Areas showing differences in grades were because it resulted with either forest areas and non-vegetation area or vegetation area and non forest area. To assess such areas, we suggested to apply the high-level land cover classification. Areas showing difference in grades between results using actual vegetation map and precise forest type map, the applicability were reviewed by comparing the results of using the 1:25,000 5th forest type map. Through the comparison of assessment results, we concluded that the precise forest type map is suitable for an alternative of biotope map in assessing the ‘Naturalness’ and ‘Stability’.