@article{ART001081458},
author={김선미 and 김재명 and 전준성 and 안준석 and 김대영 and 서재성 and 박경한 and 박정현 and 김대중 and 한장희},
title={CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2007},
volume={20},
number={3},
pages={225-233},
doi={10.11637/aba.2007.20.3.225}
TY - JOUR
AU - 김선미
AU - 김재명
AU - 전준성
AU - 안준석
AU - 김대영
AU - 서재성
AU - 박경한
AU - 박정현
AU - 김대중
AU - 한장희
TI - CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells
JO - Anatomy & Biological Anthropology
PY - 2007
VL - 20
IS - 3
PB - 대한체질인류학회
SP - 225
EP - 233
SN - 2671-5651
AB - CD98, a disulfide-linked 125-kDa heterodimeric type II transmembrane glycoprotein, regulates the
func-tions of β1 integrin, suggesting that it may play a role in tumor cell invasion. In this study, the effects of CD98
signaling on the adhesion and invasion of tumor cells were investigated.
The expression of CD98 on MCF-7 human breast carcinoma cells was confirmed by immunohistochemistry. The
effects of CD98 activation on the adhesion to extracellular matrix (ECM) and invasion of MCF-7 cells were determined
by adhesion assay and cell invasion assay. Dominant negative forms of focal adhesion kinase (FAK) were transiently
transfected into MCF-7 cells using liposome reagents.
CD98 stimulation increased the adhesion of MCF-7 cells to fibronectin, laminin and collagen IV. Activation of
CD98 augmented the invasion rate of MCF-7 cells through ECM. EDTA or a function-blocking anti-β1 integrin mAb
suppressed the effect of CD98 on invasiveness. Inhibition of phosphorylation of FAK by PP2, an inhibitor of Src family
kinase, reduced CD98-induced invasion of MCF-7 cells. This result was confirmed by over-expression of dominant
negative forms of FAK. In addition, cytochalasin D or phalloidin inhibited CD98-mediated induction of tumor cell
invasion. Inhibitory effects of PP2, cytochalasin D or phalloidin on CD98-stimulated invasion of MCF-7 cells were
diminished by pretreatment of cells with Mn++, which is shown to induce conformational change of β1 intgerin.
These results provide the first evidence that CD98 activation increases tumor cell invasion by activating β1 integrin
affinity, and that FAK phosphorylation and subsequent cytoskeletal reorganization may be essential for CD98-mediated
regulation of cell motility.
KW - CD98;β1 integrin;Tumor cell invasion;Cell-ECM adhesion;FAK;Actin;Cytoskeleton
DO - 10.11637/aba.2007.20.3.225
ER -
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중 and 한장희. (2007). CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells. Anatomy & Biological Anthropology, 20(3), 225-233.
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중 and 한장희. 2007, "CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells", Anatomy & Biological Anthropology, vol.20, no.3 pp.225-233. Available from: doi:10.11637/aba.2007.20.3.225
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중, 한장희 "CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells" Anatomy & Biological Anthropology 20.3 pp.225-233 (2007) : 225.
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중, 한장희. CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells. 2007; 20(3), 225-233. Available from: doi:10.11637/aba.2007.20.3.225
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중 and 한장희. "CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells" Anatomy & Biological Anthropology 20, no.3 (2007) : 225-233.doi: 10.11637/aba.2007.20.3.225
김선미; 김재명; 전준성; 안준석; 김대영; 서재성; 박경한; 박정현; 김대중; 한장희. CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells. Anatomy & Biological Anthropology, 20(3), 225-233. doi: 10.11637/aba.2007.20.3.225
김선미; 김재명; 전준성; 안준석; 김대영; 서재성; 박경한; 박정현; 김대중; 한장희. CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells. Anatomy & Biological Anthropology. 2007; 20(3) 225-233. doi: 10.11637/aba.2007.20.3.225
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중, 한장희. CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells. 2007; 20(3), 225-233. Available from: doi:10.11637/aba.2007.20.3.225
김선미, 김재명, 전준성, 안준석, 김대영, 서재성, 박경한, 박정현, 김대중 and 한장희. "CD98 Activation Increases the Invasion of Human Breast Carcinoma MCF-7 Cells" Anatomy & Biological Anthropology 20, no.3 (2007) : 225-233.doi: 10.11637/aba.2007.20.3.225
