Development of Ancient DNA Isolation Method for Improved PCR Amplification (고인골 유전자 증폭 효율이 높은 핵산 분리 방법)

김기정; 아리오나 턱럼; 전은희; 이민수; 조연옥; 가와치?L 르학와수렝; 최지혜; 다스제벡 투멩; 김근철; Kim, Jae-Hyun (김재현); 노맹석; 박기원; 박애자; 유권종; 김종대; 이광호; 김경용

doi:10.11637/aba.2007.20.4.267

Development of Ancient DNA Isolation Method for Improved PCR Amplification

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2007, 20(4), pp.267~281
DOI : 10.11637/aba.2007.20.4.267
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

김기정 ¹, 아리오나 턱럼 ¹, 전은희 ¹, 이민수 ¹, 조연옥 ¹, 가와치?L 르학와수렝 ¹, 최지혜 ¹, 다스제벡 투멩 ², 김근철 ³, Kim, Jae-Hyun ⁴, 노맹석 ⁵, 박기원 ⁶, 박애자 ¹, 유권종 ¹, 김종대 ¹, 이광호 ¹, 김경용 ¹

¹중앙대학교
²몽골국립대학 사회과학대학원 인류고고학부
³강원대학교 자연과학대학 생명과학부
⁴동아대학교 인문과학대학 고고미술사학과
⁵부경대학교 자연과학대학 수리과학부
⁶국립과학수사연구소

Accredited

ABSTRACT

Ancient DNA analyses are widely used for evolutionary and phylogenetic study of mankind in anthropology and archeology. However, the DNA extraction from particularly poorly preserved ancient human samples is often unsuccessful in these analyses. In the present study, to improve the success rate of ancient DNA analysis, we introduced a high grade ancient DNA purification method using ion-exchange columns. We compared the success rate of ancient DNA analysis of this new method with that of the two methods that have been used for ancient DNA extraction, GENECLEAN kit (Qbiogene) and Qiaquick column (Qiagen). Twelve ancient bone samples from Korea and Mongolia that are about 500 to 5,000 years old by an archeological estimation were used. As the DNA analysis methods, polymerase chain reaction (PCR) methods for the amplification of a mitochondrial DNA HV1 segment, a male sex determination marker DNA and M175 marker DNA that is used for the determination of O haplogroup of Y chromosome that is reportedly a common one in modern Korean people. The method developed in this study remarkably increased the success rate of DNA analysis compared with the other two methods. Using the GENECLEAN kit, only two samples were amplifiable for the mitochondrial DNA, no sam-ples for the male sex determination marker and M175 marker DNAs. Using the Qiaquick columns, nine samples were amplifiable for mitochondirial DNA, nine samples for male sex determination marker and six samples for M175 marker. The developed method allowed for the amplification of mitochondrial DNA from all samples, male sex determination marker from eight samples and M175 marker from eight samples. The results demonstrate that ion-exchange columns can be useful for the improved ancient DNA extraction in anthropology and archeology.

KEYWORDS

Ancient DNA, DNA extraction, Ion-exchange column, Polymerase chain reaction

Citation status

* References for papers published after 2023 are currently being built.

[journal] Calacal GC / 2005 / Fungal DNA challenge in human STR typing of bone samples 50 : 1394~1401

[journal] Cattaneo C / 1997 / Comparison of three DNA extraction methods on bone and blood stains up to 43 years old and amplification of three different gene sequences 42 : 1126~1135

[journal] Cattaneo C / 1995 / A simple method for extracting DNA from old skeletal material 74 : 167~174

[journal] Edson SM / 2004 / Naming the dead-confronting the realities of rapid identification of degraded skeletal remains forensic 16 : 63~90

[journal] Evison MP / 1997 / Extraction of single-copy nuclear DNA from forensic specimens with a variety of postmortem histories 42 : 1032~1038

[journal] Faerman M / 1995 / Sex identification of archaeological human remains based on amplification of the X and Y amelogenin alleles 167 : 327~332

[journal] Fischer GF / 2001 / An HLA class-II allele frequent in Eskimos and Amerindians is found in the Tyrolean Ice Man 65 : 363~369

[journal] Hagelberg E / 1991 / Isolation and characterization ofDNA from archaeological bone 244 : 45~50

[journal] H?nni C / 1995 / Isopropanol precipitation removes PCR inhibitors from ancient bone extracts 23 : 881~882

[journal] H？ss M / 1993 / DNA extraction from Pleistocene bonesby a silica-based purification method : 3913~3914

[journal] H?ss M / 1996 / DNA damage and DNA sequence retrieval from ancient tissues 24 : 1304~1307

[journal] Hunter P / 2006 / Ancient DNA research goes nuclear : A new technique to extract sequence data from nuclear DNA may reveal exciting new insights into evolution and phylogeny 7 : 136~139

[journal] Jobling MA / 2003 / The human Y chromosome: an evolutionary marker comes of age 4 : 598~612

[journal] Kalmar T / 2000 / A simple and efficient method for PCR amplifiable DNA extraction from ancient bones 28(E67)

[journal] Kemp BM / 2005 / Use of bleach to eliminate contaminating DNA from the surface of bones and teeth 154 : 53~61

[journal] Keyser-Tracqui C / 2005 / Methods for the study of ancient DNA 297 : 253~264

[journal] Kolman CJ / 2000 / Ancient DNA analysis of human populations 111 : 5~23

[journal] Kumar SS / 2000 / Brief communication: discouraging prospects for ancient DNA from India 113 : 129~133

[journal] Kurosaki K / 1993 / Individual DNA identificationfrom ancient human remains 53 : 638~643

[journal] Mulligan CJ / 2005 / Isolation and analysis of DNA from archaeological, clinical, and natural history specimens 395 : 87~103

[journal] Neeser D / 1995 / Sex determination of forensic samples by simultaneous PCR amplification of a-satellite DNA from both the X and Y chromosomes 40 : 239~241

[journal] O?rourke DH / 1996 / Ancient DNA: methods, progress, and perspectives 8 : 557~571

[journal] P狎bo S / 2004 / Genetic analyses from ancient DNA 38 : 645~679

[journal] Poinar HN / 1996 / Amino acid racemization and the preservation of ancient DNA 272 : 864~866

[journal] Prado VF / 1997 / Extraction of DNA from human skeletal remains: practical applications in forensic sciences 14 : 41~44

[journal] Rohland N / 2007 / Comparison and optimization of ancient DNA extraction 42 : 343~352

[journal] Tebbe CC / 1993 / Interference of humic acids and DNAextracted directly from soil in detection and transformationof recombinant DNA from bacteria and a yeast 59 : 2657~2665

[journal] Underhill PA / 2001 / The phylogeography of Y chromosome binary haplotypes and the origins of modern human populations 65 : 43~62

[journal] Walsh PS / 1991 / Chelex 100 as a mediumfor simple extraction of DNA for PCR-based typing fromforensic material 10 : 506~513

[journal] Yang DY / 1998 / Technical note: improved DNA extraction from ancient bones using silica-based spin columns 105 : 539~543

[journal] Zaporozhenko EV / 2006 / Method for rapid DNA extraction from bacterial communities of different soils 75 : 127~134

KJCKorea
Journal Central

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Development of Ancient DNA Isolation Method for Improved PCR Amplification

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Development of Ancient DNA Isolation Method for Improved PCR Amplification

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (4)

REFERENCES (31) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.