@article{ART001231053},
author={양성준 and KIM MIN JEONG and Ho Joong Jeong and Ghi Chan Kim and 길영기 and 김강련 and Kim， Hyun-su},
title={Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={1},
pages={21-29},
doi={10.11637/aba.2008.21.1.21}
TY - JOUR
AU - 양성준
AU - KIM MIN JEONG
AU - Ho Joong Jeong
AU - Ghi Chan Kim
AU - 길영기
AU - 김강련
AU - Kim， Hyun-su
TI - Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 1
PB - 대한체질인류학회
SP - 21
EP - 29
SN - 2671-5651
AB - The ubiquitin-proteasome system is crucial in maintaining cellular growth and metabolism. Dysfunction of this system may contribute to neurodegenerative diseases, such as Parkinson’s disease. But its effects on primary neurons are largely unknown.
In the present study, we investigated the effects of proteasome inhibitor and hypoxia on primary neuronal cultures to determine whether proteasomal malfunction induces neuronal death.
Neuronal apoptosis increased in primary cultured cortical neurons with treatment of proteasomal inhibitor in normoxic condition and in the presence or absence of proteasomal inhibitor in hypoxic condition. Also expression of PARP and activated caspase 3 increased. NF-κB, a key transcription factor in this system expression increased in hypoxic condition and proteasomal inhibition. Interestingly, hypoxic condition induced an expression and accumulation of α-synuclein in neuron, one of components of Lewy body in Parkinson’s disease.
Our findings determine that hypoxic condition may affect the ubiquitin-proteasome system. Furthermore, it suggests that hypoxic condition and proteasomal inhibitors are involved, at least in parts, in neurodegeneration of mouse model for Parkinson’s disease.
KW - Primary cultured cortical neuron;Hypoxia;Proteasomal inhibitor;α-synuclein;NF-κB
DO - 10.11637/aba.2008.21.1.21
ER -
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련 and Kim， Hyun-su. (2008). Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures. Anatomy & Biological Anthropology, 21(1), 21-29.
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련 and Kim， Hyun-su. 2008, "Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures", Anatomy & Biological Anthropology, vol.21, no.1 pp.21-29. Available from: doi:10.11637/aba.2008.21.1.21
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련, Kim， Hyun-su "Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures" Anatomy & Biological Anthropology 21.1 pp.21-29 (2008) : 21.
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련, Kim， Hyun-su. Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures. 2008; 21(1), 21-29. Available from: doi:10.11637/aba.2008.21.1.21
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련 and Kim， Hyun-su. "Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures" Anatomy & Biological Anthropology 21, no.1 (2008) : 21-29.doi: 10.11637/aba.2008.21.1.21
양성준; KIM MIN JEONG; Ho Joong Jeong; Ghi Chan Kim; 길영기; 김강련; Kim， Hyun-su. Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures. Anatomy & Biological Anthropology, 21(1), 21-29. doi: 10.11637/aba.2008.21.1.21
양성준; KIM MIN JEONG; Ho Joong Jeong; Ghi Chan Kim; 길영기; 김강련; Kim， Hyun-su. Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures. Anatomy & Biological Anthropology. 2008; 21(1) 21-29. doi: 10.11637/aba.2008.21.1.21
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련, Kim， Hyun-su. Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures. 2008; 21(1), 21-29. Available from: doi:10.11637/aba.2008.21.1.21
양성준, KIM MIN JEONG, Ho Joong Jeong, Ghi Chan Kim, 길영기, 김강련 and Kim， Hyun-su. "Effects of Hypoxia on the Ubiquitin-proteasome Systemin Primary Cortical Neuronal Cell Cultures" Anatomy & Biological Anthropology 21, no.1 (2008) : 21-29.doi: 10.11637/aba.2008.21.1.21
