Protective Effect of HSP90 on Neuronal Cell Death-inducedby β-Amyloid Peptide (아밀로이드 베타에 의한 신경세포 사멸과 이에 대한열충격 단백질 90의 세포 보호효과)

이현정; 김도희; 김옥현; Chung Yoon Hee (정윤희); 김경용; 김성수; 이원복

doi:10.11637/aba.2008.21.1.31

Protective Effect of HSP90 on Neuronal Cell Death-inducedby β-Amyloid Peptide

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2008, 21(1), pp.31~40
DOI : 10.11637/aba.2008.21.1.31
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

이현정 ¹, 김도희 ¹, 김옥현 ¹, Chung Yoon Hee ¹, 김경용 ¹, 김성수 ¹, 이원복 ¹

¹중앙대학교

Withdraw

이 논문은 대한체질인류학회 윤리위원회 회의(2020.2.27.) 결과 일부 그림이나 내용이 중복된 점이 확인되어 게재가 철회된 논문임

ABSTRACT

In the present study, we determined the protective mechanism of HSP90 against neuronal cell death induced by Aβ. For the evaluation of protective role of HSP90, we used human neuroblastoma SK-N-SH cell lines, examined AlamarBlue assay, Western blot analysis and immunofluorescence assay. Incubation of SK-N-SH cells with Aβ significantly induced neuronal cell death. However, HSP90 induced by mild heat shock could attenuate neuronal apoptosis in Aβ treated condition. To identify the role of HSP90, we determined localization of HSP90 in SK-N-SH cells. Interestingly, HSP90 was increased and localized in mitochondria as treatment of mild heat shock. Also, treatment or increase of HSP90 largely elevated level of Bcl-2 expression, whereas inhibition of HSP90 with HSP90 antisense oligonucleotide significantly decreased Bcl-2 expression. In contrast to Bcl-2, Bax expression was regulated independently by HSP90. Moreover, increase of HSP90 could attenuate collapse of mitochondrial membrane potential induced by Aβ. However, HSP90 antisense oligonucleotide largely increase breakdown of mitochondrial membrane potential induced by Aβ. These data suggest that HSP90 as chaperone protein significantly attenuates neuronal damage and protects neuroanl cells from neurotoxin such as Aβ.

KEYWORDS

Amyloid beta (Aβ), HSP90, Mitochondria, Bcl-2, Bax, Apoptosis

Citation status

* References for papers published after 2023 are currently being built.

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Protective Effect of HSP90 on Neuronal Cell Death-inducedby β-Amyloid Peptide

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* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Protective Effect of HSP90 on Neuronal Cell Death-inducedby β-Amyloid Peptide

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