@article{ART001231054},
author={조지연 and 서윤경 and 전수경 and 백두진},
title={Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={1},
pages={41-54},
doi={10.11637/aba.2008.21.1.41}
TY - JOUR
AU - 조지연
AU - 서윤경
AU - 전수경
AU - 백두진
TI - Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 1
PB - 대한체질인류학회
SP - 41
EP - 54
SN - 2671-5651
AB - Remote ischemic preconditioning (IP), brief tolerating cycles of ischemia and reperfusion in remote non-vital organs,
can reduce ischemic injury of the heart. IP induces cardiac protection by down-regulating iNOS or up-regulating eNOS.
In addition, Akt has been known to protect myocardium against ischemia-reperfusion injury. This study was undertaken
to observe the expression of iNOS, eNOS, Akt and phospho-Akt (p-Akt) in the rat myocardium after IP.
Thirty-five weeks-old male Sprague-Dawley rats were divided into control and IP groups. The IP group was further
subdivided into 3 groups based on the number of cycles of IP. For IP, left commom iliac artery was occluded 3, 6 and
10 cycles for 5 min of ischemia alternating with 5 min of reperfusion. The rat were sacrificed at 0, 3, 6, 24 and 72 hours
of IP and the heart was removed. The expression of iNOS, eNOS, Akt and p-Akt in the rat myocardium was examined
by immunohistochemical staining and Western blot analysis.
The expression of iNOS was increased by IP and was higher in 10IP groups than 3IP and 6IP group. The expression
of eNOS was increased or decreased by IP and was showed no difference with increasing episode of IP. The expression
of Akt was decreased by IP at 24 and 72 hours after reperfusion, and showed no differences with increasing episode of
IP. The expression of p-Akt was increased by IP and showed no difference with increasing episode of IP.
These results suggest that hind limb ischemic preconditioning provides cardiac protection through up-regulation of
eNOS and phosphorylation of Akt, however excessive episodes of remote preconditioning may induce the myocardial
ischemic injury through overexpression of iNOS.
KW - Remote ischemic preconditioning;Rat;Myocardium;iNOS;eNOS;Akt;p-Akt
DO - 10.11637/aba.2008.21.1.41
ER -
조지연, 서윤경, 전수경 and 백두진. (2008). Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium. Anatomy & Biological Anthropology, 21(1), 41-54.
조지연, 서윤경, 전수경 and 백두진. 2008, "Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium", Anatomy & Biological Anthropology, vol.21, no.1 pp.41-54. Available from: doi:10.11637/aba.2008.21.1.41
조지연, 서윤경, 전수경, 백두진 "Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium" Anatomy & Biological Anthropology 21.1 pp.41-54 (2008) : 41.
조지연, 서윤경, 전수경, 백두진. Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium. 2008; 21(1), 41-54. Available from: doi:10.11637/aba.2008.21.1.41
조지연, 서윤경, 전수경 and 백두진. "Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium" Anatomy & Biological Anthropology 21, no.1 (2008) : 41-54.doi: 10.11637/aba.2008.21.1.41
조지연; 서윤경; 전수경; 백두진. Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium. Anatomy & Biological Anthropology, 21(1), 41-54. doi: 10.11637/aba.2008.21.1.41
조지연; 서윤경; 전수경; 백두진. Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium. Anatomy & Biological Anthropology. 2008; 21(1) 41-54. doi: 10.11637/aba.2008.21.1.41
조지연, 서윤경, 전수경, 백두진. Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium. 2008; 21(1), 41-54. Available from: doi:10.11637/aba.2008.21.1.41
조지연, 서윤경, 전수경 and 백두진. "Effects of Remote Ischemic Preconditioning on the Expressionsof NOS and Akt in the Rat Myocardium" Anatomy & Biological Anthropology 21, no.1 (2008) : 41-54.doi: 10.11637/aba.2008.21.1.41
