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@article{ART001231055},
author={박재현 and 김규천 and 곽현호 and In-Ryoung Kim and 이승은 and 정 진 and 박혜련 and 신상훈 and 김철훈 and 남창옥 and 박봉수 and 조수현},
title={Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2008},
volume={21},
number={1},
pages={55-68},
doi={10.11637/aba.2008.21.1.55}

TY - JOUR
AU - 박재현
AU - 김규천
AU - 곽현호
AU - In-Ryoung Kim
AU - 이승은
AU - 정 진
AU - 박혜련
AU - 신상훈
AU - 김철훈
AU - 남창옥
AU - 박봉수
AU - 조수현
TI - Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells
JO - Anatomy & Biological Anthropology
PY - 2008
VL - 21
IS - 1
PB - 대한체질인류학회
SP - 55
EP - 68
SN - 2671-5651
AB - Chios gum mastic (CGM) is obtained from the stem and leaves of Pistacia lentiscus trees and has been extensively used for centuries in Mediterranean and Middle Eastern countries, both as a dietary supplement and herbal remedy. This study was undertaken to examine in vitro effects of cytotoxicity and growth inhibition, and the molecular mechanism underlying modulation of cell cycle and induction of apoptosis in YD9 human oral squamous carcinoma cell line treated with CGM. The viability of YD9 cells and human normal keratinocyes (HaCaT cells), and the growth inhibition of YD9 cells were assessed by the MTT assay and clonogenic assay respectively. The hoechst staining and DNA electrophoresis were conducted to observe the YD9 cells undergoing apoptosis. YD9 cells were treated with CGM, and Western blotting, immunocytochemistry, confocal microscopy and FACScan flow cytometry were conducted. Mitochondrial membrane potential change and proteasome activity were measured. CGM treatment on YD9 cells resulted in a does-dependent inhibition of cell growth and induced apoptotic cell death. And tested YD9 cells showed several lines of apoptotic manifestation. Flow cytometric analysis revealed that CGM resulted in G1 arrest in cell cycle progression which was associated with decrease in the protein expression of cyclin D1, cyclin D3, Cdk2 and Cdk4, and increase in the protein expression of p21WAF1/CIP1 and p53. These results demonstrate that CGM induces G1 the cell cycle arrest via the modulation of cell cycle-related proteins, and apoptosis via mitochondria and caspase pathway in YD9 cells, suggesting that CGM can be considered as a novel therapeutic strategy for human oral squamous cell carcinoma from its strong cell cycle arrest and apoptosis-inducing activity.
KW - Chios gum mastic (CGM);Apoptosis;Human oral squamous carcinoma cell line;Cell cycle arrest
DO - 10.11637/aba.2008.21.1.55
ER -

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수 and 조수현. (2008). Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells. Anatomy & Biological Anthropology, 21(1), 55-68.

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수 and 조수현. 2008, "Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells", Anatomy & Biological Anthropology, vol.21, no.1 pp.55-68. Available from: doi:10.11637/aba.2008.21.1.55

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수, 조수현 "Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells" Anatomy & Biological Anthropology 21.1 pp.55-68 (2008) : 55.

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수, 조수현. Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells. 2008; 21(1), 55-68. Available from: doi:10.11637/aba.2008.21.1.55

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수 and 조수현. "Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells" Anatomy & Biological Anthropology 21, no.1 (2008) : 55-68.doi: 10.11637/aba.2008.21.1.55

박재현; 김규천; 곽현호; In-Ryoung Kim; 이승은; 정 진; 박혜련; 신상훈; 김철훈; 남창옥; 박봉수; 조수현. Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells. Anatomy & Biological Anthropology, 21(1), 55-68. doi: 10.11637/aba.2008.21.1.55

박재현; 김규천; 곽현호; In-Ryoung Kim; 이승은; 정 진; 박혜련; 신상훈; 김철훈; 남창옥; 박봉수; 조수현. Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells. Anatomy & Biological Anthropology. 2008; 21(1) 55-68. doi: 10.11637/aba.2008.21.1.55

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수, 조수현. Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells. 2008; 21(1), 55-68. Available from: doi:10.11637/aba.2008.21.1.55

박재현, 김규천, 곽현호, In-Ryoung Kim, 이승은, 정 진, 박혜련, 신상훈, 김철훈, 남창옥, 박봉수 and 조수현. "Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis inYD9 Human Oral Squamous Carcinoma Cells" Anatomy & Biological Anthropology 21, no.1 (2008) : 55-68.doi: 10.11637/aba.2008.21.1.55