Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors

Lee, Young (이영); 홍동균; 최대경; 임슬기; 손경철; Im Myung (임명); Seo Young Joon (서영준); Lee， Young Ho (이영호); 이중훈; Kim Chang Deok (김창덕)

doi:10.11637/aba.2014.27.2.71

Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2014, 27(2), pp.71~77
DOI : 10.11637/aba.2014.27.2.71
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

Lee, Young ¹, 홍동균 ¹, 최대경 ¹, 임슬기 ¹, 손경철 ¹, Im Myung ², Seo Young Joon ¹, Lee， Young Ho ¹, 이중훈 ¹, Kim Chang Deok ¹

¹충남대학교
²충남대학교병원

Accredited

ABSTRACT

O-linked β-N-acetylglucosamine modification is an important post-translational modification,emerging as a novel regulatory mechanism in various cellular events. Recently, several studies have shown thatO-GlcNAcylation plays an essential role in human breast, lung, and colon cancers. With regard to skin cancers,the role of O-GlcNAcylation has yet to be elucidated. To investigate whether O-GlcNAcylation is linked tohuman skin tumor development, immunohistochemical analysis was performed to investigate the presence of OGlcNAcylationin various skin tumors. We evaluated the levels of O-GlcNAcylation, O-GlcNAc transferase, andO-GlcNAcase in 29 benign tumors, 12 premalignant tumors, and 26 malignant tumors in skin. Compared to thebenign tumors, premalignant and malignant tumors had increased patterns of O-GlcNAcylation. In addition, theO-GlcNAc transferase and O-GlcNAcase levels were higher in premalignant and malignant tumors than in benigntumors. Interestingly, O-GlcNAcase levels were significantly increased in premalignant tumors compared tobenign and malignant tumors. These results suggest that O-GlcNAcylation of proteins may play an important rolein the development of human skin tumors.

KEYWORDS

Immunohistochemistry, O-GlcNAc, O-GlcNAcase, O-GlcNAc transferase, Skin neoplasm

Citation status

* References for papers published after 2023 are currently being built.

[journal] Torres CR / 1984 / Topography and polypeptide distribution of terminal N-acetylglucosamine residues on the surfaces of intact lymphocytes / J Biol Chem 259 : 3308~3317

[journal] Hart GW / 2007 / Cycling of O-linked beta-N-acetylglucosamine on nucleocytoplasmic proteins / Nature 446 : 1017~1022

[journal] Kreppel LK / 1997 / Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats / J Biol Chem 272 : 9308~9315

[journal] Gao Y / 2001 / Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic β-N-acetylglucosaminidase from human brain / J Biol Chem 276 : 9838~9845

[journal] Kang JG / 2009 / OGlcNAc protein modification in cancer cells increases in response to glucose deprivation through glycogen degradation / J Biol Chem 284 : 34777~34784

[journal] Gu Y / 2010 / GlcNAcylation plays an essential role in breast cancer metastasis / Cancer Res 70 : 6344~6351

[journal] Mi W / 2011 / O-Glc-NAcylation is a novel regulator of lung and colon cancer malignancy / Biochim Biophys Acta 1812 : 514~519

[journal] Snow CM / 1987 / Monoclonal antibodies identify a group of nuclear pore complex glycoproteins / J Cell Biol 104 : 1143~1156

[journal] Caldwell SA / 2010 / Nutrient sensor O-GlcNAc transferase regulates breast cancer tumorigenesis through targeting of the oncogenic transcription factor FoxM1 / Oncogene 29 : 2831~2842

[journal] Lynch TP / 2012 / Critical role of O-Linked β-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis / J Biol Chem 287 : 11070~11081

[journal] Yehezkel G / 2012 / Olinked β-N-acetylglucosaminylation (O-GlcNAcylation) in primary and metastatic colorectal cancer clones and effect of N-acetyl-β-D-glucosaminidase silencing on cell phenotype and transcriptome / J Biol Chem 287 : 28755~28769

[journal] Zhu Q / 2012 / O-Glc-NAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation / Med Oncol 29 : 985~993

[journal] Ma Z / 2013 / Hyper-O-GlcNAcylation is anti-apoptotic and maintains constitutive NF-κB activity in pancreatic cancer cells / J Biol Chem 288 : 15121~15130

[journal] Vosseller K / 2002 / Diverse regulation of protein function by O-GlcNAc: a nuclear and cytoplasmic carbohydrate post-translational modification / Curr Opin Chem Biol 6 : 851~857

[journal] Hanover JA / 2012 / Bittersweet memories:linking metabolism to epigenetics through O-GlcNAcylation / Nat Rev Mol Cell Biol 13 : 312~321

[journal] Slawson C / 2010 / O-GlcNAc signaling:a metabolic link between diabetes and cancer? / Trends Biochem Sci 35 : 547~555

[journal] Ma Z / 2013 / -GlcNAc in cancer biology / Amino Acids 45 : 719~733

[journal] Yang WH / 2006 / Modification of p53 with O-linked N-acetylglucosamine regulates p53 activity and stability / Nat Cell Biol 8 : 1074~1083

KJCKorea
Journal Central

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors

ABSTRACT

KEYWORDS

Citation status

* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors

ABSTRACT

KEYWORDS

Statistics

Tools

Issue List

Citation status

KCI Citation Counts (0)

REFERENCES (18) * References for papers published after 2023 are currently being built.

Search PDF

Citation

* References for papers published after 2023 are currently being built.