Multiplex Ligation Dependent Probe Amplification (MLPA) Assay on Joseon Mummified Samples from Archaeological Sites of South Korea (조선시대 미라 뇌 조직을 이용한 질병 관련 유전자 진단에서 Multiplex Ligation Dependent Probe Amplification Assay 기법적용 가능성 검토)

김유수; Chang Seok Oh (오창석); Jong Ha Hong (홍종하); 성문우; Shin, Dong Hoon (신동훈)

doi:10.11637/aba.2015.28.3.137

Multiplex Ligation Dependent Probe Amplification (MLPA) Assay on Joseon Mummified Samples from Archaeological Sites of South Korea

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2015, 28(3), pp.137~143
DOI : 10.11637/aba.2015.28.3.137
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy

김유수 ¹, Chang Seok Oh ², Jong Ha Hong ¹, 성문우 ³, Shin, Dong Hoon ²

¹서울대학교 의과대학 해부학교실
²서울대학교
³서울대학교 의과대학 검사의학교실

Accredited

ABSTRACT

Multiplex Ligation dependent Probe Amplification (MLPA) to detect large deletions or duplications has been widely used as a diagnostic tool for various disease clinically. As this method requires only a small amount of template DNA and is very simple and high throughput, it has numerous advantages for the analysis of the human specimen obtained from archaeological sites. In this study we therefore tried to perform MLPA analysis for detecting any of duplications or deletions in mummy samples (n=4) from medieval Joseon tombs of Korea. Of them, we could not get any authentic data from 3 samples by MLPA method while only one case (HD2) showed the possible presence of duplications or deletions during her lifetime. Although the current report reveal that MLPA is a promising tool for anthropological study in South Korea, more studies are still needed to make up for the validity problem of commercial MLPA kit used in this study.

KEYWORDS

Mummification, MLPA, Korea

Citation status

* References for papers published after 2023 are currently being built.

[journal] 기호철 / 2014 / Infection patterns of trematode parasites among Joseon people / 대한의사협회지 / 대한의사협회 57(10) : 866~875

[journal] Min Seo / 2014 / Paleoparasitological studies on mummies of the Joseon Dynasty, Korea. / The Korean Journal of Parasitology / 대한기생충학ㆍ열대의학회 52(3) : 235~242

[journal] Oh CS. / 2013 / Amplification of DNA remnants in mummified human brains from medieval Joseon tombs of Korea / Anthropol Anz 70 : 57~81

[journal] 김이석 / 2013 / Evidence of Periostitis in Joseon Dynasty Skeletons / 대한체질인류학회지 / 대한체질인류학회 26(2) : 81~90

[confproc] Oh CS. / 2012 / Autosomal short tandem repeat analysis of ancient DNA by coupled use of mini-and conventional STR kits / J Forensic Sci 57 : 820~825

[journal] 신동훈 / 2011 / Enterobius vermicularis Eggs Discovered in Coprolites from a Medieval Korean Mummy / The Korean Journal of Parasitology / 대한기생충학ㆍ열대의학회 49(3) : 323~326

[journal] 이우영 / 2002 / Anthropometry of a Male Child Mummy from a Tomb of Joseon Dynasty Period in Korea / 대한체질인류학회지 / 대한체질인류학회 15(4) : 263~278

[confproc] Cheng S. / 1994 / Effective amplification of long targets from cloned inserts and human genomic DNA / Proc Natl Acad Sci 91 : 5695~5699

[journal] Kim GH. / 2009 / MLPA application in genetic testing / J Genet Med 6 : 146~154

[journal] den Dunnen JT / 2006 / MLPA and MAPH: Sensitivedetection of deletions and duplication / Curr Protoc Hum Genet

[confproc] Roberts RG. / 1992 / Point mutations in the dystrophin gene / Proc Natl Acad Sci USA 89 : 2331~2335

[journal] 조한나 / 2010 / Clinical Usefulness of Molecular Diagnosis in Dystrophin Gene Mutations Using the Multiplex Ligation-dependent Probe Amplification (MLPA) Method / 대한신경과학회지 / 대한신경과학회 28(1) : 22~26

[journal] Hardy J. / 2006 / Genetics of Parkinson’s disease and parkinsonism / Ann Neurol 60 : 389~398

[journal] Gerdes T. / 2008 / Multiplex ligation-dependent probe amplification(MLPA)in prenatal diagnosis-experience of a large series of rapid testing for aneuploidy of chromosomes 13, 18, 21, X and Y / Prenat Diagno 28 : 1119~1125

[journal] Roselló M. / 2010 / Prenatal study of common submicroscopic"genomic disorders"using MLPA with subtelomeric/microdeletaion syndrome probe mixes, among gestations with ultrasound abnormalities in the first trimester / Eur J Med Genet 53 : 76~79

[journal] Bunyan DJ. / 2004 / Dosage analysis of cancer predisposition genes by multiplex ligation-dependent probe amplification / Br J Cancer 91 : 1155~1159

[journal] Bittel DC. / 2007 / Methylation-specific multiplex ligation-dependent probe amplification anaylsis of subjects with chromosome 15 abnormalities / Genet Test 11 : 467~477

[journal] Procter M / 2006 / Molecular diagnosis of Prader-Willi and Angelman syndromes by methylation-specific melting analysis and methylation-specific multiplex ligation-dependent probe amplification / Clin Chem 52 : 1276~1283

[web] MRC Holland / Product description P275-C2-0213 MAPTGRN-v07 / http://www.mlpa.com

[web] MRC Holland / MLPA DNA protocol version MDP-v003. Available at http://www.mlpa.com / http://www.mlpa.com

[journal] Gillessen-Kaesbach G. / 1995 / Genotype-phenotype correlation in a series of 167 deletion and non-deletion patients with Prader-Willi syndrome / Hum Genet 96 : 638~643

[journal] Schouten JP / 2002 / Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification / Nucleic Acids Res 30 : 57~

[journal] Kozlowski P. / 2008 / New application and developments in the use of multiplex ligation-dependent probe amplification / Eletrophoresis 29 : 4627~4636

[journal] Sorensen KM. / 2012 / Screening of congenital heart disease patients using Multiplex ligation-dependent probe amplification : early diagnosis of syndromic patients / Am J Med Genet 158A : 720~725

[journal] 조은해 / 2009 / Comparing Two Diagnostic Laboratory Tests for Several Microdeletions Causing Mental Retardation Syndromes: Multiplex Ligation-Dependent Amplification vs Fluorescent In Situ Hybridization / Annals of Laboratory Medicine / 대한진단검사의학회 29(1) : 71~76

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Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Multiplex Ligation Dependent Probe Amplification (MLPA) Assay on Joseon Mummified Samples from Archaeological Sites of South Korea

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* References for papers published after 2023 are currently being built.

Anatomy & Biological Anthropology 2023 KCI Impact Factor : 0.33

Multiplex Ligation Dependent Probe Amplification (MLPA) Assay on Joseon Mummified Samples from Archaeological Sites of South Korea

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