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A comparative study of primary cilia length in renal tubules using scanning electron microscopy

  • Anatomy & Biological Anthropology
  • Abbr : Anat Biol Anthropol
  • 2024, 37(4), pp.279~286
  • DOI : 10.11637/aba.2024.37.4.279
  • Publisher : 대한체질인류학회
  • Research Area : Medicine and Pharmacy > Anatomy
  • Received : October 1, 2024
  • Accepted : December 2, 2024
  • Published : December 31, 2024

지미진 1 뭉궁치메그 1 송수진 1 이수연 1 박소현 1 Jung Mi Han 2 Ki Hwan Han 1

1이화여자대학교 의과대학 해부학교실
2이화여자대학교 의과대학 나노이미징 실험실

Accredited

ABSTRACT

The primary cilium is a hair-like structure extending from the luminal surface of kidney tubular cells. Its microstructure and function in the kidney are still not fully understood. This study aimed to detect and compare primary cilia across various renal tubules. Normal adult Sprague-Dawley rats were perfused with 2% periodate-lysine-paraformaldehyde fixative and processed for light and electron microscopy. Antibodies against acetylated α-tubulin and aquaporin 1 were used to label primary cilia and proximal tubules, respectively. Primary cilia were largely concealed among the microvilli in proximal tubules, making accurate observation challenging under electron microscopy. No significant differences were found in the lengths of primary cilia in the distal tubules (2.1 ± 0.22 μm) and collecting ducts (1.86 ± 0.31 μm). However, primary cilia in the thin limbs of the loop of Henle were significantly longer, measuring 4.2 ± 0.73 μm, compared to those in adjacent distal tubules and collecting ducts. These findings demonstrate that the primary cilia in the thin limbs are the longest in the normal kidney, suggesting they may have a distinct function in this segment.

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