A comparative study of primary cilia length in renal tubules using scanning electron microscopy (콩팥 세관에서 주사전자현미경을 이용한 일차섬모의 길이 비교 연구)

Mi Jin Jee (지미진); Mungunchimeg Chultemsuren (뭉궁치메그); Soo-Jin Song (송수진); Su-Youn Lee (이수연); So-Hyun Park (박소현); Jung-Mi Han (한정미); Ki-Hwan Han (한기환)

doi:10.11637/aba.2024.37.4.279

A comparative study of primary cilia length in renal tubules using scanning electron microscopy

Anatomy & Biological Anthropology
Abbr : Anat Biol Anthropol
2024, 37(4), pp.279~286
DOI : 10.11637/aba.2024.37.4.279
Publisher : 대한체질인류학회
Research Area : Medicine and Pharmacy > Anatomy
Received : October 1, 2024
Accepted : December 2, 2024
Published : December 31, 2024

Mi Jin Jee ¹, Mungunchimeg Chultemsuren ¹, Soo-Jin Song ¹, Su-Youn Lee ¹, So-Hyun Park ¹, Jung-Mi Han ², Ki-Hwan Han ¹

¹이화여자대학교 의과대학 해부학교실
²이화여자대학교 의과대학 나노이미징 실험실

Accredited

ABSTRACT

The primary cilium is a hair-like structure extending from the luminal surface of kidney tubular cells. Its microstructure and function in the kidney are still not fully understood. This study aimed to detect and compare primary cilia across various renal tubules. Normal adult Sprague-Dawley rats were perfused with 2% periodate-lysine-paraformaldehyde fixative and processed for light and electron microscopy. Antibodies against acetylated α-tubulin and aquaporin 1 were used to label primary cilia and proximal tubules, respectively. Primary cilia were largely concealed among the microvilli in proximal tubules, making accurate observation challenging under electron microscopy. No significant differences were found in the lengths of primary cilia in the distal tubules (2.1 ± 0.22 μm) and collecting ducts (1.86 ± 0.31 μm). However, primary cilia in the thin limbs of the loop of Henle were significantly longer, measuring 4.2 ± 0.73 μm, compared to those in adjacent distal tubules and collecting ducts. These findings demonstrate that the primary cilia in the thin limbs are the longest in the normal kidney, suggesting they may have a distinct function in this segment.

KEYWORDS

Primary cilia, Renal tubules, Thin limbs, Electron microscopy

Citation status

* References for papers published after 2024 are currently being built.

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This paper was written with support from the National Research Foundation of Korea.

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Anatomy & Biological Anthropology 2024 KCI Impact Factor : 0.36

A comparative study of primary cilia length in renal tubules using scanning electron microscopy

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* References for papers published after 2024 are currently being built.

Anatomy & Biological Anthropology 2024 KCI Impact Factor : 0.36

A comparative study of primary cilia length in renal tubules using scanning electron microscopy

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