The purpose of this study was to develop a video intervention tool for psychological stabilization by verifying differences in psychophysiological indicators (positive affect, negative affect, state anxiety, EEG, HRV) in response to video stimulation. Twelve university students were randomly assigned to the group “Only” where participants watched single-typed video stimuli or to the group “Mix” where they watched mixed-typed video stimuli. Before and after each video stimulus, the participants completed the PANAS scale and STAI-X scale, and HRV and EEG was recorded during the video stimulation. For the group “Only”, a total score of positive and negative affect, an individual score of interested , irritable , inspired , nervous, attentive , enthusiastic , and active , and the brain index such as ‘RT’ at Fp1, Fp2, and T5, ‘RA’ and ‘RSA’ at F7, ‘RG’ at Fp1, Fp2 and T3, ‘RHB’ at C3 and C4, ‘RAHB’ at Fz showed significant difference between video stimuli. For the group “Mix”, significant differences were found in a total score of positive and negative affect, interested, nervous, hostile, afraid, state anxiety, and brain index such as ‘RSA’ at P3 and Fz, ‘RHB’ and ‘RAHB’ at P3, ‘RFA’ at F8 and O2, ‘RLB’ at F8, F4 and P4, ‘RMB’ at Fp2, ‘RST’ at Cz, and asymmetry between P3 and P4. No significant difference was found for HRV.