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Development of an Eco-Friendly Biochar-Based Nanobiosensor for Real-Time Detection of Sweat Molecules

  • Industry Promotion Research
  • Abbr : IPR
  • 2025, 10(4), pp.171~177
  • DOI : 10.21186/IPR.2025.10.4.171
  • Publisher : Industrial Promotion Institute
  • Research Area : Interdisciplinary Studies > Interdisciplinary Research
  • Received : September 27, 2025
  • Accepted : October 27, 2025
  • Published : October 31, 2025

Min Hyuk Kang 1 Nayun Park 1 Jimin Paik 1 Soo Bin Shin 1 Songhee Lee 1 Seo Hyun Jung 1 Jae Min Choi 1 Dong Sup Kim 1 Ja Hyun Lee 1

1순천향대학교

Accredited

ABSTRACT

Seaweed, recognized as a sustainable bioresource, is increasingly applied across various industries. However, the treatment of residual seaweed sludge generated during processing remains a persistent challenge. In this study, biochar derived from pyrolyzed Undaria pinnatifida (brown algae) sludge was utilized as an electron transfer mediator to develop an electrochemical nanobiosensor capable of real-time glucose detection in sweat. The fabricated sensor exhibited a linear current response across biochar concentrations ranging from 0.0001% to 0.2%, and cyclic voltammetry analysis revealed that the oxidation and reduction peak currents increased proportionally with glucose concentration. The optimal enzymatic activity was observed at a glucose oxidase (GOD) concentration of 0.1 g/L, where maximum current output was recorded. Furthermore, the sensor demonstrated excellent selectivity and stability even in the presence of common interfering substances such as lactic acid and urea. Compared to conventional carbon nanotubes (CNTs), the seaweed-derived biochar offered an eco-friendly and cost-effective alternative with high electrical conductivity. This study highlights a sustainable sensor platform that not only addresses waste reduction but also contributes to the advancement of real-time health monitoring technologies.

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