The purpose of this study is to offer the basic data and method for assessing the naturalinessof temple environment by applying the ecological index of plant to quantitatively and objectivelyassess the flora and to understand the flora distributed in major temple forest. The ecological index of plant was based in the surveyed flora. The whole flora were summarized as 535 taxa including 103families, 310 genera, 426 species, 5 subspecies, 82 varieties and 22 forms. And, the numbers of taxa inthe Beopheungsa were 267 taxa, 314 taxa of the Daeseungsa and 296 taxa of the Seongjusa. The rareplants were 9 taxa including Aristolochia contorta, Viola albida, Arisaema heterophyllumand so forth, andthe Korean endemic plants were 11 taxa including Clematis trichotoma, Stewartia pseudocamellia, Viciachosenensis and so forth. The specific plants by floristic region were 51 taxa including Wistaria floribunda,Celtis aurantiaca, Angelica gigas and so forth, and the pteridophyta were 26 taxa including Osmundajaponica, Polystichum braunii, Onoclea orientalis and so forth. The naturalized plants were 35 taxaincluding Fallopia dumetorum, Conyza canadensis, Xanthium strumarium and so forth, the invasive alienplants were 3 taxa including Ambrosia artemisiifolia, Aster pilosus and Eupatorium rugosum. In the resultsof analysing the ecological index of plant in whole temple forests, RI(Rare Index) was 1.7%, 2.1% ofEI(Endemic Index), 9.5% of SI(Specific Index), 1.2 of PI(Pteridophyta Index) and 6.5% of NI(NaturalizedIndex) in narrow sense. In broad sense, FI(Flora Index) was 11.0%, 1.5% of RI, 3.4% of EI, 4.8% of SI,10.1% of PI, 10.9% of UI(Urbanized Index) and 25.0% of DI(Disturbed Index). The FI of the Daeseungsawas the highest among the three temple forests. In narrow sense, the RI, SI, NI and DI of theBeopheungsa was high, the seongjusa was higher the EI and SI. In broad sense, the RI and SI of theBeopheungsa, the EI, SI and PI of the Seongjusa, the UI is high in the Daeseungsa. In future, if we willanalyze ecological index of plant in other temple forests, we will offer the help to establishing the planof conservation and restoration on ecosystem in whole temple forests.Environmental Specimen Banks(ESBs) are playing pivotal role in monitoring the effect of environmental pollution on the ecosystembased on the retrospective analysis of the representative samples collected regularly and stored incryogenic condition.