Purpose: To investigate the differences between genders in university students in Yanbian, China, regarding their experiences, knowledge, and attitude toward sex. Methods: This was a cross-sectional structured questionnaire survey using a convenient sampling method. Data was collected using an online survey via mobile -phones or computer devices. Results: There were 287 students who completed the survey, with 64.9% of them female and 35.1% male. Among sexual experiences, the amount of sexual intercourse (x2 =4.70, p=.030), students' feelings after their first sexual intercourse (x2 =10.79, p=.005), and their preferred contraceptive method (x2 =7.67, p=.025) differed by gender. No significant difference between genders was shown regarding the level of knowledge about sex. The average score of correct answers was 53.7%. Male students showed significantly higher scores on attitudes toward sexuality than females (t=8.65, p<.001). Attitudes toward sexuality differed -between the genders in all the demographic characteristics, except for an understanding of sexual education, where female and males alike were lacking. Conclusion: The results show that among university students, both genders show a lack of knowledge about sexuality, and that attitudes toward sex differ according to gender. This has important implications for comprehensive, gender-equal sex education in universities, and should focus especially on artificial abortion and providing accurate contraceptive methods.