@article{ART001142785},
author={이희상 and 남궁기 and 육기환 and 안준석 and 노맹석 and 김대진 and Chung Yoon Hee and 김성수 and 이원복 and 김경용},
title={Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2006},
volume={19},
number={3},
pages={223-233},
doi={10.11637/aba.2006.19.3.223}
TY - JOUR
AU - 이희상
AU - 남궁기
AU - 육기환
AU - 안준석
AU - 노맹석
AU - 김대진
AU - Chung Yoon Hee
AU - 김성수
AU - 이원복
AU - 김경용
TI - Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier
JO - Anatomy & Biological Anthropology
PY - 2006
VL - 19
IS - 3
PB - 대한체질인류학회
SP - 223
EP - 233
SN - 2671-5651
AB - Vitamin E is the most important lipid-soluble antioxidant in humans. Although alpha-tocopherol is suggested that it
has protective effect from many diseases, little is known about the prevention of occludin alteration in tight junction of
blood-brain barrier (BBB) under pathologic insults producing reactive oxygen species (ROSs).
In this study, the effects of alpha-tocopherol on H2O2-induced tight junction occludin were studied. Primary culture
of rat brain microvessel endothelial cells was investigated with confocal microscopy, Western blot, and cell viability assay.
Alpha-tocopherol had no apparent cytotoxicity up to 2.8 mM. The preincubation with alpha-tocopherol suppressed
the H2O2-induced cytotoxicity in Alamar Blue assay and phase contrast microscopy. In confocal laser microscopy and
Western blot, H2O2-induced loss of occludin was suppressed by preincubation with alpha-tocopherol.
The present findings provide evidence that alpha-tocopherol may be beneficial for cellular protection from
pathologic insults. Since alpha-tocopherol was demonstrated to have far fewer adverse effects, it would become a
noteworthy nutrient or drug for the treatment of neurodegenerative diseases.
KW - Alpha-tocopherol;BBB;Tight junction;Occludin;H2O2
DO - 10.11637/aba.2006.19.3.223
ER -
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복 and 김경용. (2006). Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier. Anatomy & Biological Anthropology, 19(3), 223-233.
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복 and 김경용. 2006, "Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier", Anatomy & Biological Anthropology, vol.19, no.3 pp.223-233. Available from: doi:10.11637/aba.2006.19.3.223
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복, 김경용 "Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier" Anatomy & Biological Anthropology 19.3 pp.223-233 (2006) : 223.
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복, 김경용. Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier. 2006; 19(3), 223-233. Available from: doi:10.11637/aba.2006.19.3.223
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복 and 김경용. "Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier" Anatomy & Biological Anthropology 19, no.3 (2006) : 223-233.doi: 10.11637/aba.2006.19.3.223
이희상; 남궁기; 육기환; 안준석; 노맹석; 김대진; Chung Yoon Hee; 김성수; 이원복; 김경용. Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier. Anatomy & Biological Anthropology, 19(3), 223-233. doi: 10.11637/aba.2006.19.3.223
이희상; 남궁기; 육기환; 안준석; 노맹석; 김대진; Chung Yoon Hee; 김성수; 이원복; 김경용. Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier. Anatomy & Biological Anthropology. 2006; 19(3) 223-233. doi: 10.11637/aba.2006.19.3.223
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복, 김경용. Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier. 2006; 19(3), 223-233. Available from: doi:10.11637/aba.2006.19.3.223
이희상, 남궁기, 육기환, 안준석, 노맹석, 김대진, Chung Yoon Hee, 김성수, 이원복 and 김경용. "Alpha-tocopherol Prevents H2O2-induced Tight Junction Occludin Disruption in Blood-Brain Barrier" Anatomy & Biological Anthropology 19, no.3 (2006) : 223-233.doi: 10.11637/aba.2006.19.3.223
