@article{ART001913258},
author={Guanghai Yan and Choi Yun Ho},
title={Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats},
journal={Anatomy & Biological Anthropology},
issn={2671-5651},
year={2014},
volume={27},
number={3},
pages={113-122},
doi={10.11637/aba.2014.27.3.113}
TY - JOUR
AU - Guanghai Yan
AU - Choi Yun Ho
TI - Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats
JO - Anatomy & Biological Anthropology
PY - 2014
VL - 27
IS - 3
PB - 대한체질인류학회
SP - 113
EP - 122
SN - 2671-5651
AB - Sparassis crispa is an edible mushroom with various medicinal properties. Here we demonstrate theeffect of Sparassis crispa on carbon tetrachloride (CCl4)-induced hepatotoxicity and the underlying mechanism.
To evaluate the hepatoprotective effects of Sparassis crispa ethanol extract (SCE), 50 male Sprague-Dawleyrats were equally divided into 5 groups. Group I is the normal control rats with an intraperitoneal (i.p.) 0.5%carboxy methyl cellulose (CMC) pretreatment and olive oil treatment. Group II is the model group with an i.p.
0.5% CMC and 0.5 mL/kg CCl4 treatment. Group III and IV is the CCl4-administered rats pretreated with an i.p.
100 and 200 mg/kg SCE, respectively. Group V includes the silymarin group with an i.p. 50 mg/kg silymarin andCCl4 treatment.
At 16 h after the CCl4 treatment, the levels of serum aminotransferases, TNF-α, and lipid peroxidation weresubstantially increased, whereas the activity of hepatic antioxidative enzymes, such as superoxide dismutase andcatalase, was decreased. These changes were attenuated by SCE. The histological studies also showed that SCEinhibited the CCl4-induced liver injury. Furthermore, the contents of hepatic nitrite, inducible nitric oxide synthase(iNOS), and cyclooxygenase-2 (COX-2) were elevated after CCl4 treatment, while the cytochrome P450 2E1(CYP2E1) expression was suppressed. SCE treatment inhibited the formation of liver nitrite, reduced the overexpressionof iNOS and COX-2 proteins, but restored the liver CYP2E1 content compared with the CCl4-treatedmodel group.
The present data elucidate that SCE protects the liver against CCl4-induced acute hepatotoxicity, which mightbe due to its ability to restore the CYP2E1 function and suppress the inflammatory responses, in combination withits capacity to reduce oxidative stress.
KW - Sparassis crispa;Carbon tetrachloride;Hepatotoxicity
DO - 10.11637/aba.2014.27.3.113
ER -
Guanghai Yan and Choi Yun Ho. (2014). Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats. Anatomy & Biological Anthropology, 27(3), 113-122.
Guanghai Yan and Choi Yun Ho. 2014, "Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats", Anatomy & Biological Anthropology, vol.27, no.3 pp.113-122. Available from: doi:10.11637/aba.2014.27.3.113
Guanghai Yan, Choi Yun Ho "Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats" Anatomy & Biological Anthropology 27.3 pp.113-122 (2014) : 113.
Guanghai Yan, Choi Yun Ho. Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats. 2014; 27(3), 113-122. Available from: doi:10.11637/aba.2014.27.3.113
Guanghai Yan and Choi Yun Ho. "Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats" Anatomy & Biological Anthropology 27, no.3 (2014) : 113-122.doi: 10.11637/aba.2014.27.3.113
Guanghai Yan; Choi Yun Ho. Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats. Anatomy & Biological Anthropology, 27(3), 113-122. doi: 10.11637/aba.2014.27.3.113
Guanghai Yan; Choi Yun Ho. Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats. Anatomy & Biological Anthropology. 2014; 27(3) 113-122. doi: 10.11637/aba.2014.27.3.113
Guanghai Yan, Choi Yun Ho. Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats. 2014; 27(3), 113-122. Available from: doi:10.11637/aba.2014.27.3.113
Guanghai Yan and Choi Yun Ho. "Sparassis crispa Attenuates Carbon Tetrachloride-Induced Hepatic Injury in Rats" Anatomy & Biological Anthropology 27, no.3 (2014) : 113-122.doi: 10.11637/aba.2014.27.3.113
