Reference resources management tools can be divided into three types: Desktop-based, Web-based, and Web 2.0-based. This study is intended to compare and analyze the functions provided by the three types of tools and also to compare and evaluate their usability. Each Tools were chosen to represent each type. For comparison and analysis of the functions provided by the three types of tools, we divided the process of using reference resources management tools into four steps and then compared and analyzed sub items in each step. Satisfaction, understandability, conformance and efficiency that items for comparison and evaluation of the usability were compared using both quantitative and qualitative approaches. We surveyed the participants to collect data for quantitative research; and interviewed them and analyzed the results for qualitative research. Here are the results from evaluating the usability of Tool A(Desktop-based), B(Web-based), C(Web 2.0 based). First, Tool B had the highest mean user satisfaction, followed by Tool A and Tool C. Secondly, Tool B had the lead in understandability, followed by Tool A and Tool C. Thirdly, Tool A had the highest mean conformance, followed by Tool B and Tool C. Fourth, Tool B had the highest mean efficiency, followed by Tool A and Tool C.